Adams, James N. (University of South Dakota, Vermillion). Recombination between Nocardia erythropolis and Nocardia canicruria. J. Bacteriol. 88:865-876. 1964.-Nutritionally complementary auxotrophic mutants derived from Nocardia erythropolis did not yield prototrophic recombinants when progeny from mixed cultures or newly mixed strains were inoculated onto minimal medium. Similarly, complementa...

The ubiquitous bacterial biofilm regulator, c-di-GMP can form G-quadruplexes at physiological conditions in the presence of some aromatic compounds, such as acriflavine and proflavine. The fluorescence of these compounds is quenched upon c-di-GMP binding and some of the formed c-di-GMP G-quadruplexes are stable even at 75 °C.

The Mycobacterium smegmatis genome contains many genes encoding putative drug efflux pumps. Yet with the exception of lfrA, it is not clear whether these genes contribute to the intrinsic drug resistance of this organism. We showed first by reverse transcription (RT)-PCR that several of these genes, including lfrA as well as the homologues of Mycobacterium tuberculosis Rv1145, Rv1146, Rv1877, R...

Genetic studies of the determination of Salmonella flagella have suggested that the alternative antigenic phases correspond to two distinct homology groups, i.e., to factors at two distinct genetic loci, one for phase 1 ("specific phase"), and one for phase 2 ("group phase") (Lederberg and Edwards, 1953). It has, however, been generally understood that the two phases were equivalent to one anot...

a strength of 1:500 with distilled water, and the process of staining is carried out exactly in the same way as that described under the above method, except that acriflavine in the abovementioned strength is used instead of methylene blue. I use the counter-stain for half to one minute. To obtain the best results, all the detail in the careful preparation and fixation of smears, as well as the...

A novel drug efflux gene (named sepA, staphylococcal efflux pump gene) is cloned from antiseptic-resistant mutants of Staphylococcus aureus into Escherichia coli. The sepA gene conferred the reduction of susceptibility to acriflavine and the acceleration of ethidium bromide efflux from the E. coli cells. The sepA (474 bp) encoded the protein that has four predicted transmembrane segments. These...

with 5% glucose, but without agar, was used in all growth and inhibition studies. Davis and Mingioli (J. Bacteriol. 60:17, 1950) synthetic liquid medium supplemented with 0.4% sodium glutamate was used for inoculum preparation. Inocula were prepared from cells of single colonies. Aerobic growth studies were carried out in a water-bath shaker at 35 C and anaerobic studies in Brewer jars. Compara...

In the title compound, C(13)H(10)N(+)·C(7)H(5)O(3) (-) or (acrH)(+)(Hsal)(-), the asymmetric unit contains one acridinium cation and one salicylate anion. The acridinium N atom is protonated and the carb-oxy-lic acid group of salicylic acid is deprotonated. Both moieties are planar, with an r.m.s. deviation of 0.0127 Å for the acr cation and 0.0235 ° for the sal anion. They are aligned with a d...