Rabbit antibodies were prepared against the major hemoglobin components of the larval and adult stages of R. catesbeiana . The properties of the antisera were studied by double immunodiffusion, precipitation, and complement fixation . The antisera to tadpole and frog hemoglobins did not cross-react with either hemoglobin or apohemoglobin . The antiserum against frog hemoglobin was used for the ...

Rabbit antibodies were prepared against the major hemoglobin components of the larval and adult stages of R. catesbeiana. The properties of the antisera were studied by double immunodiffusion, precipitation, and complement fixation. The antisera to tadpole and frog hemoglobins did not cross-react with either hemoglobin or apohemoglobin. The anti-serum against frog hemoglobin was used for the de...

Induction of fetal hemoglobin (HbF) has therapeutic importance for patients with beta-hemoglobin disorders. Previous studies showed that let-7 microRNAs (miRNAs) are highly regulated in erythroid cells during the fetal-to-adult developmental transition, and that targeting let-7 mediated the up-regulation of HbF to greater than 30% of the total globin levels in human adult cultured erythroblasts...

The J2E erythroid cell line, generated by transforming fetal liver cells, terminally differentiates in response to erythropoietin (epo). The cells expressed both adult and embryonic globin genes, although considerably more adult globin was produced, and transcripts for both species rose following exposure to epo. A 6-fold increase in transcription of the adult alpha and beta maj globin genes wa...

Here we investigated in primary human erythroid tissues a downstream element of the heterochronic let-7 miRNA pathway, the insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1), for its potential to affect the hemoglobin profiles in human erythroblasts. Comparison of adult bone marrow to fetal liver lysates demonstrated developmental silencing in IGF2BP1. Erythroid-specific overexpressi...

Rabbit antibodies were prepared against the major hemoglobin components of the larval and adult stages of R. catesbeiana . The properties of the antisera were studied by double immunodiffusion, precipitation, and complement fixation . The antisera to tadpole and frog hemoglobins did not cross-react with either hemoglobin or apohemoglobin . The antiserum against frog hemoglobin was used for the ...

In the course of the study on the hemoglobin contained in newborn bovine blood, two major and five minor components which are specific to newborn bovine hemoglobin were detected by the methods of agar-gel electrophoresis, cellulose-acetate membrane electrophoresis and column chromatography. These components were examined on their alkaline resistance , and the results showed that all the compone...

OBJECTIVE We assessed the association between gender and mortality on antiretroviral therapy (ART) using identical models with and without sex-specific categories for weight and hemoglobin. DESIGN Cohort study of adult patients on ART. SETTING GHESKIO Clinic in Port-au-Prince, Haiti. PARTICIPANTS 4,717 ART-naïve adult patients consecutively enrolled on ART at GHESKIO from 2003 to 2008. ...