The downstream processing of proteins remains the most significant cost in protein production, and is largely attributed to rigorous chromatographic purification protocols, where the stringency of purity for biopharmaceutical products sometimes exceeds 99%. With an ever burgeoning biotechnology market, there is a constant demand for alternative purification methodologies, to ameliorate the depe...

We present an inertial microfluidic platform for rapid, multiplexed affinity purification of proteins and cells from complex mixtures. Beads of different sizes, coated with different bait molecules bind specific proteins or cells in a single reaction step and the bound targets are then size-sorted by flowing in a spiral microchannel. We use this scheme to simultaneously purify antibodies specif...

Affinity chromatography has been demonstrated to be a powerful tool for the isolation and purification of parasite proteins and has potential applications for diagnosis and therapy. Many studies have focused on parasite proteins that modulate host cell defense, as gp63, a glycoprotein from Leishmania spp., that is involved in the cleavage of the complement factor C3b to iC3b, which promotes adh...

From among a wide variety of protein purification techniques affinity chromatography has proved to be particularly effective for separation of proteolytic enzymes and their inhibitors. In this article, following a general description of affinity adsorbents used for purification of proteinases, we overview a simple separation procedure for some serine proteinases and their inhibitors by way of a...

A new method for the preparation of the glycoforms of bovine alpha(1)-acid glycoprotein (AGP) is described relying on affinity-reversed micellar extraction and separation (ARMES). This method has proven effective in separating structurally similar glycoproteins and separating glycoproteins from nonglycosylated proteins from natural sources. In this method, individual glycoforms complex with the...