aflatoxin m1 (afm1), a carcinogenic substance is found in milk and dairy products. the effect of season and type of dairy products on afmi level in northern iran was investigated in this study.three hundred samples (each season 75 samples) including raw and pasteurized milk, yoghurt, cheese, and cream samples were collected from three distinct milk producing farms. the samples were subjected to...

this study was carried out to detect the presence of aflatoxin m1 (afm1) in 30 uht flavored milk samples in karaj, alborz province of iran. high-performance liquid chromatography (hplc) was applied to analyse afm1 in the samples. result showed that aflatoxin m1 was detected in all the uht flavored milk samples, the concentration of afm1 was ranged from 0.015 to 0.14 µg/kg. also, 10 samples (33....

a model capable of predicting the minimum content of aflatoxin m1 (afm1) in iranian while brine cheese has been developed using a chemometric approach to determine the optimum processing conditions. renneting temperature, cut size, stirring time, press time, curd size and saturated brine ph were all regarded as process variables. three-dimensional response surface and contour plots were drawn. ...

conclusions afm1 contamination of milk is not a concern in this city. however, dairy cattle feed samples of various livestocks must be regularly checked for aflatoxin and the storage conditions of feeds should be strictly controlled. results afm1 was detected in all milk samples. the mean concentration of afm1 was 11.61 ± 0.72 ng/l with a range of 6.3-23.3 ng/l. none of the samples had levels e...

BACKGROUND Aflatoxin M1 (AFM1) is the principal hydroxylated AFB1 metabolite present in milk of cows fed with a diet contaminated with AFB1and excreted within 12 hours of administration of contaminated feeds. OBJECTIVE This study was initiated to assess the knowledge and practices of urban dairy farmers and feed millers about aflatoxin in feeds and milk, determine the prevalence and quantify ...

Because thin-layer chromatographic (TLC) confirmation of identity and reverse-phase liquid chromatographic (LC) determination with fluorescence detection of aflatoxin M1 both require the derivative formed in the reaction of M1 and trifluoroacetic acid (TFA), various reaction conditions were studied to obtain complete derivative formation. Of the various organic solvents tested, the reaction bet...

A high-affinity IgM monoclonal antibody specific for aflatoxins was covalently bound to Sepharose 4B and used as a preparative column to isolate aflatoxin derivatives from the urine of people and experimental animals who had been exposed to the carcinogen environmentally or under laboratory conditions. Aflatoxin levels were quantified by radioimmunoassay and high-performance liquid chromatograp...