Cell culture from vials usually includes an open phase performed under laminar flow hoods. Even if disposable and small closed systems are available, at least the first cell culture step after vial thawing, which is a centrifugation, is performed in disposable but open containers. This step is critical as the risk of contamination is high and it also has a direct impact on process timelines. In...

OBJECTIVE To study the resistance of human spermatozoa to cryoinjury in repeated cycles of thaw-refreezing by using the fast liquid nitrogen vapor method. MATERIALS AND METHODS Semen specimens were obtained from sixteen normal and oligozoospermic individuals who required disposal at the sperm bank. Five of them had testicular cancer. Specimens were thawed and an aliquot was removed for analys...

objective: the aim of the present study was to evaluate three thawing rates on the post thaw motility, viability and chromatin structure of buffalo semen frozen in 0.5-ml straws. materials and methods: in this experimental study semen was collected with artificial vagina (42˚c) from four buffalo bulls.split pooled ejaculates (n=4) were extended at 37˚c with a bioxcell® extender. semen was coole...

Currently, the assessment of sperm function in a raw or processed semen sample is not able to reliably predict sperm ability to withstand freezing and thawing procedures and in vivo fertility and/or assisted reproductive biotechnologies (ART) outcome. The aim of the present study was to investigate which parameters among a battery of analyses could predict subsequent spermatozoa in vitro fertil...

Objective Vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. However, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. This study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, mor...

BACKGROUND Successful cryopreservation of rat spermatozoa from various strains still remains a challenge. The objective of this study was to determine if combinations of OptiPrep™ (iodixanol) and adenosine 5'-triphosphate (ATP) can improve rat sperm function during the cryopreservation procedure. METHODS Epididymal rat spermatozoa were frozen under different OptiPrep™ concentrations (0, 1, 2,...