Intracellular free Ca2+ [( Ca2+]i) and pH (pHi) were measured simultaneously by dual wavelength excitation in thrombin-stimulated human platelets double-labeled with the fluorescent probes fura2 and 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein to determine the relationship between changes in [Ca2+]i and pHi, respectively. At 37 degrees C, thrombin (0.5 or 0.1 units/ml) increased [Ca2+]i with ...

Tubular fluid flow, urine osmolality, and pH were selectively altered to determine the relative protective roles of these factors in a rat model of acute urate nephrophathy. Various prehyper uricemic conditions were established in five groups of animals: (a) normopenic Wistar rats given no pretreatment (Group I); (b) Wistar rats given acetazolamide, 20 mg/kg, and isotonic NaHCO3 to produce urin...

We used microelectrodes to examine the effects of organic substrates, particularly lactate (Lac-), on the intracellular pH (pHi) and basolateral membrane potential (Vbl) in isolated, perfused proximal tubules of the tiger salamander. Exposure of the luminal and basolateral membranes to 3.6 mM Lac- caused pHi to increase by approximately 0.2, opposite to the decrease expected from nonionic diffu...

Globally, land alkalinization affecting agricultural development. Considering the increasingly serious effects of alkaline stress on agriculture and environment, phytoremediation may be an efficient way to addressed alkalinization. Broomcorn millet (Panicum miliaceum L.) is a saline-alkaline-tolerant crop bioenergy crop. However, molecular mechanism response broomcorn remains large gap. To expl...

Free fatty acids, such as oleic acid, are able to act as uncouplers of oxidative phosphorylation in mitochondria [l]. They can also transport cations across membranes and may have a physiological role in iron transport in the gut [2]. We have previously shown that oleic acid can act as a K+/H+ ion exchange carrier in liposomes [3]. It was proposed that oleic acid is able to transport H+ and K+ ...

The objective of the present study was to determine if exposure of cervical mucus to air during specular examination could modify mucus pH. Detection of changes is justified because of their possible interference with sperm-mucus interaction, since an acidic pH is unfavorable to sperm penetration and is associated with infertility due to the cervical factor. Twenty women with good quality mucus...