Eleven different preparations of influenza virus were used to vaccinate large groups of human beings. The antibody response to these vaccines was measured by means of the in vitro agglutination inhibition test, and the geometric mean titers of sera taken 2 weeks after vaccination were compared. From these comparisons the following conclusions were drawn: 1. There was a wide individual variation...

Placental transfer of penicillin and gentamicin occurs both in normal pregnant mares and mares with placentitis. Microdialysis is a useful technique for monitoring therapeutic drug levels in the equine allantoic fluid and should aid development of effective treatment protocols for mares with high-risk pregnancies.

Untargeted metabolic profiling of body fluids in experimental animals and humans exposed to chemicals may reveal early signs of toxicity and indicate toxicity pathways. Avian embryos develop separately from their mothers, which gives unique possibilities to study effects of chemicals during embryo development with minimal confounding factors from the mother. In this study we explored blood plas...

The molecular structure of a sulfated glycoprotein from chick chorioallantoic fluid has been investigated. The effect of alkali and alkali-borohydride under various conditions has been shown to lead to quantitatively and qualitatively different products of elimination and reduction. The carbohydrate chains of the products, by gel filtration, were heterogeneous, ranging from an average molecular...

Noninfectious hemagglutinins were prepared from the allantoic fluids of embryonated chicken eggs infected with Sindbis virus or with Murray Valley encephalitis virus.

This report describes a RIA for porcine retinol binding protein (RBP) that has been validated for measurement of RBP concentrations as low as 10 ng/mL in serum, follicular fluid, uterine flushings, allantoic fluid, and endometrial and uterine tissue culture medium. The increasing displacement of porcine [125I]RBP resulting from decreasing dilutions of each sample was parallel to the standard cu...

Since the isolation of tobacco mosaic virus in the form of a characteristic, crystalline material of high molecular weight (1, 2), a wealth of experimental data has been accumulated (3-7) which indicates that tobacco mosaic virus is, in fact, a nucleoprotein, the molecules or particles of which are 15 by 280 rnp in size, with an anhydrous weight approximately 40 million times that of the hydrog...

Macromolecular material was isolated from normal allantoic fluid by a centrifugation procedure comparable to that currently employed for the concentration and purification of influenza viruses. The yield of material was found to vary with the age of the embryo, reaching a maximum average value after 14 days of incubation at 39 degrees C. of about 0.02 mg. per ml. of allantoic fluid. The purifie...

Preparations of influenza-infected chick chorio-allantoic membrane made by two types of tissue culture and by sectioning, have been studied in the electron microscope. Comparisons have been made of influenza A' (FM1), influenza A (PR8), and swine influenza (V15), three strains which produce different relative numbers of filaments. Normal surface projections which may be confused with influenza ...