Oxidation of 2’-deoxyguanosine (dG) results in the major two-electron product 8oxo-7,8-dihydro-2’-deoxyguanosine (dOG) that can undergo further oxidation to other products. One such product is 5-guanidinohydantoin (dGh) that is highly mutagenic and has been found in vivo. The ring architecture of dGh is similar to allantoin, an oxidation product of uric acid. Allantoin establishes equilibrium b...

The synthesis of (14)C-labeled xanthine/hypoxanthine, uric acid, allantoin, allantoic acid, and urea from [8-(14)C]guanine or [8-(14)C]hypoxanthine, but not from [8-(14)C]adenine, was demonstrated in a cell-free extract from N(2)-fixing nodules of cowpea (Walp.). The (14)C recovered in the acid/neutral fraction was present predominantly in uric acid and allantoin (88-97%), with less than 10% of...

it has been investigated the in vitro antioxidant properties of ethanol extracts of the contained-allantoin plants in this study. contained-allantoined plant samples plantago lanceolata, plantago major, robinia pseudoacacia, platanus orientalis and aesculus hippocastanum were tested at different concentrations. the antioxidant activities of plant samples were analysed by 1,1- diphenyl-2-picrylh...

Samples of stem exudate and plant tissue collected from field-grown soybean (Glycine max [L.] Merr.) plants were analyzed for allantoin and allantoic acid. Nitrogen in nitrate plus amino acids exceeded ureide N concentration in stem exudate prior to flowering. During all of reproductive development (from about 40 days after planting until maturity), ureide N concentration was two to six times g...

Metabolic pathways are largely conserved in eukaryotes, but the transcriptional regulation of these pathways can sometimes vary between species; this has been termed "rewiring." Recently, it has been established that in the Saccharomyces lineage starting from Naumovozyma castellii, genes involved in allantoin breakdown have been genomically relocated to form the DAL cluster. The formation of th...

BACKGROUND We used doxorubicin-based chemotherapy as a clinical model of oxidative assault in humans. METHODS The study recruited newly diagnosed breast cancer patients (n = 23). Urine samples were collected immediately before (T0) and at 1 hour (T1) and 24 hours (T24) after i.v. administration of treatment. Measurements included allantoin and the isoprostanes iPF(2alpha)-III, iPF(2alpha)-VI,...

Saccharomyces cerevisiae can degrade allantoin in five steps to glyoxylate, ammonia, and "CO(2)." We previously demonstrated that synthesis of the urea carboxylase-allophanate hydrolase multienzyme complex is contingent upon the presence of allophanic acid, the product of the urea carboxylase reaction. Since these enzymes catalyze the last two reactions of allantoin degradation, experiments wer...