Acute monocytic leukemia (AML M5 or AMoL) is one of the several types of leukemia that are still awaiting cures. The use of chemotherapy for cancer management can be harmful to normal cells in the vicinity of the target leukemia cells. This study assessed the potency of the extracts from lesser galangal, turmeric, and ginger against AML M5 to use the suitable fractions in neutraceuticals. Aqueo...

Microbial transformations are key processes in marine phosphorus cycling. In this study, we investigated the contribution of phototrophic and heterotrophic groups to phosphate (Pi) uptake fluxes in the euphotic zone of the lowPi Mediterranean Sea and estimated Pi uptake kinetic characteristics. Surface soluble reactive phosphorus (SRP) concentrations were in the range of 6–80 nmol L across the ...

A microfluidic system to determine hydrogen peroxide (H(2)O(2)) in individual HepG2 cells based on the electrokinetic gated injection was developed for the first time. A home-synthesized fluorescent probe, bis(p-methylbenzenesulfonate)dichlorofluorescein (FS), was employed to label intracellular H(2)O(2) in the intact cells. On a simple cross microchip, multiple single-cell operations, includin...

Since the start of COVID-19 outbreak, more than four million people have died this disease. Given its ability to provide a precise response, mass spectrometry-based proteomics could represent useful tool study pathology. To end, an untargeted nLC-ESI-MS/MS-based method characterise SARS-CoV-2 proteins, including possible variants, and investigate human saliva plasma proteome in single analysis ...

Leukocytes from patients with acute granulocytic leukemia (AGL), acute monocytic leukemia (AMoL) and blastic crisis of chronic granulocytic leukemia (BC) were incubated with serine-U-C’4 or aspartic acid-U-C’4. The metabolism was followed by determination of radioactivity of CO , lipids, proteins, organic acids, and nucleotides. Leukemic leukocytes in AGL, AMoL, and BC exhibit high serine utili...

Peripheral blood leukemia cells from patients with acute monoblastic leukemia (AMoL) were tested for killer cell activity against target cells that detected natural killer cell-mediated or monocyte-mediated spontaneous cytotoxicity. The fibrosarcoma cell line Wehi 164, pretreated with actinomycin D to induce susceptibility to lysis, specifically detects the activity of unstimulated human monocy...

The monoclonal antibody solution hybridization assay is a novel enzyme immunoassay for detection of RNA with a biotinylated DNA probe. To increase the sensitivity of this test, a fluorescent substrate and an enzymatic amplification cycling system were compared with a conventional colorigenic substrate for alkaline phosphatase. The fluorescent, cycling, and colorigenic substrates detected, respe...

Leukemic blasts from patients with acute nonlymphoid leukemia were examined for the presence of Ig, receptors for IgGFc, and for their capacity to mediate antibody-dependent cellular cytotoxicity (ADCC) against chicken red blood cells (RBC) coated with IgG and spontaneous cell-mediated cytotoxicity (SCMC) against cells of K562 cell line. Leukemic blasts from acute myeloblastic leukemia (AML) pa...

We attempted to apply fluorescence resonance energy transfer technology to nucleic acid sequence-based amplification (FRET-NASBA) on the platform of the LightCycler system to detect Aspergillus species. Primers and probes for the Aspergillus 18S rRNA were newly designed to avoid overlapping with homologous sequences of human 18s rRNA. NASBA using molecular beacon (MB) showed non-specific result...

In this issue, Zou et al. report that vitamin C abrogated the ability of bortezomib (PS-341, Velcade) to induce apoptosis, G2-M arrest, and cleavage of caspase 8, caspase 3, and poly(ADP-ribose) polymerase and to augment tumor necrosis factor–related apoptosis-inducing ligand–induced apoptosis in various human cancer cell lines (1). Moreover, vitamin C suppressed bortezomib-mediated inhibition ...