Muffin et al. (1), in this issue, describe a more specific method for pancreatic ainylase in serum. Amylase of salivary origin is precipitated with a monoclonal antibody, and the amylase remaining is presumed to come from the pancreas. It is likely that their test will be more specific for pancreatic disease. Since the recognition of multiple sources of antylase and of the frequency of hyperamy...

New ELISAs for detecting macroamylase or free autoantibodies to amylase were tested with 48 samples that had been characterized by gel chromatography and electrophoresis. The macroamylase ELISA, with anti-IgG or anti-IgA for detection, detected macroamylase in 28 of 33 samples known to contain macroamylase (85% sensitivity), whereas the ELISA for free autoantibody to amylase was positive for on...

The impact of ultra violet radiations on alpha amylase producing ability of Bacillus subtilis (2620) was studied. Maximum enzymatic activity (crude) upto 17 folds were obtained from mutant strain exposed to 60 minutes ultra violet irradiation in comparison to parent strain. Purified enzyme showed molecular weight of 68 KDa and 54 KDa by SDS-PAGE in both parent and mutant strains. The SDS-PAGE o...

Streptococcus mutans has been implicated as the major causative agent of human dental caries. S. mutans binds to saliva-coated tooth surfaces, and previous studies suggested that fimbriae may play a role in the initial bacterial adherence to salivary components. The objectives of this study were to establish the ability of an S. mutans fimbria preparation to bind to saliva-coated surfaces and d...

Variation in the ability of Aspergillus flavus isolates to spread between cotton boll locules was previously shown to be at least partially related to variation in the production of a specific polygalacturonase (pectinase P2C). To determine if non-pectolytic hydrolase differences between low- and high-virulence isolates exist and, thus, could also potentially contribute to virulence differences...

Understanding the relationships between biochemical contents and morpho-phenological aspects of a plant during life cycle would be helpful for doing precise agricultural practices through its cultivation. In the present study, various biochemical parameters were evaluated in relation to bulb storage, shoot growth and florogenesis in Iranian lily (Lilium ledebourii var. Kelardasht Salehi1). Ten ...

BACKGROUND We present the case of an 80-year-old woman who was admitted to hospital with an intermittent volvulus of the right colon. A total colectomy was performed. Initially, serum amylase and lipase increased concordantly, but after a few weeks amylase normalized (85 U/L), whereas lipase increased to 3764 U/L. This discrepancy and persistence of hyperlipasemia suggested a macromolecular for...

The wheat proteins soluble in chloroform-methanol mixtures are associated with several kinds of food allergies. A separation method based on capillary zone electrophoresis (CZE) with UV detection was developed for the analysis of these mixtures. An acidic phosphoric acid/β-alanine (pH 2.5) buffer containing HPMC, urea and acetonitrile was used for the separation. The capillary electrophoresis (...