Eco-enzymes are one of the potential sources for obtaining amylolytic bacterial isolates. The study aims to screen bacteria from eco-enzymes, characterize semi-purification amylase, and identify molecularly using 16S rRNA gene. Screening was carried out by measuring index on a Nutrient agar medium containing 1% tapioca starch. isolate which had highest non-pathogenic selected amylase characteri...

The present investigation deals with the purification and characterization of enzyme α-amylase from a mutant strain of Bacillus licheniformis EMS-6. A laboratory scale stirred fermentor of 7.5 L capacity was used for the enzyme production under optimal conditions. The enzyme was purified up to homogeneity level by Ammonium sulphate and ion-exchange chromatography using a fast protein liquid chr...

Amylase is one of the most important enzymes widely used in paper, textile, pharmaceutical industries etc. In the present investigation a thermostable, alkalophilic amylase was isolated from underlying soil samples of domestic wastes, and was screened on starch agar medium. The amylase activity was measured using colorimetric method at 540nm. Among forty five isolates, thirteen demonstrated cle...

An alkaliphilic and highly thermostable α-amylase producing Bacillus sp. was isolated from Van soda lake. Enzyme synthesis occurred at temperatures between 25°C and 40°C. Analysis of the enzyme by SDS-PAGE revealed a single band which was estimated to be 66 kDa. The enzyme was active in a broad temperature range, between 20°C and 90°C, with an optimum at 50°C; and maximum activity was at pH 10....

A new lipolytic strain of Bacillus sp. was isolated from oil contaminated soil sample. On measurement of biochemical parameters and 16S rDNA sequence information the strain was identified as Bacillus pumilus RK31 (NCBI accession no GQ463238). The lipase purification steps involved, 60% ammonium sulphate saturation, gel filtration chromatography using Sephadex G200 and ion exchange chromatograph...

BACKGROUND Traditional agro-systems in arid areas are a bulwark for preserving soil stability and fertility, in the sight of "reverse desertification". Nevertheless, the impact of desert farming practices on the diversity and abundance of the plant associated microbiome is poorly characterized, including its functional role in supporting plant development under drought stress. METHODOLOGY/PRI...

Thermostable α-amylase is of great importance in the starch fermentation industry; it is extensively used in the manufacture of beverages, baby foods, medicines, and pharmaceuticals. Bacillus amyloliquefaciens produces thermostable α-amylase; however, production of thermostable α-amylase is limited. Ion-beam implantation is an effective method for mutation breeding in microbes. We conduct...

Isolation and identification of thermophilic Bacillus sp was carried out from a soil sample. The cells were cultivated in a medium containing soluble starch as sole carbon source. The addition of calcium (10 mM) or peptone (1%) and yeast extract (0.5%) to the medium shortened the lag period and improved the growth and amylase synthesis. The optimum temperature for amylase production was detecte...

Di CARLO, F., AND REDFERN, S. 1947 Alpha-amylase from Bacillus subtilis. I. Purification and physical properties. Arch. Biochem., 15, 333-342. DINGLE, T., REID, W. W., AND SOLOMONS, G. L. 1953 The enzymatic degradation of pectin and other polysaccharides. II. Application of the "cup-plate" assay to the estimation of enzymes. J. Sci. Food Agr., 3, 149-155. GOODMAN, T. T. 1950 Adaptive production...