Complication of peritonitis developed in a 61 year old patient who was undergoing continuous ambulatory peritoneal dialysis (CAPD) and whose dialysate culture was negative for both aerobic and anaerobic bacteria and fungi. Despite the administration of various antibiotics and repeated mechanical lavages, inflammatory response and fever could not be controlled and therefore on the 8th hospital d...

This paper describes the use of a quantitative competitive polymerase chain reaction (QC-PCR) assay; using PCR primers to the rRNA locus of rumen fungi and a standard-control DNA including design and validation. In order to test the efficiency of this method for quantifying anaerobic rumen fungi, it has been attempted to evaluate this method in in vitro conditions by comparing with an assay bas...

An extensive in vitro study was conducted to delineate the influence of levels and source of sulfur besides rumen fungi habitat on the degradation of pearl millet stover dry matter and fibrous constituents. The results revealed that degradability of dry matter, organic matter, acid detergent fibre, neutral detergent fibre and cellulose from pearl millet stover was maximized (P<0.05), irrespecti...

Establishing the phylogeny of fungi and protists often has proved difficult owing to the simple morphologies and convergent characters in these organisms. We used DNA sequences of nuclear small-subunit ribosomal RNA genes to determine phylogenetic relationships among three major classes of organisms considered to be fungi--Basidiomycetes, Ascomycetes and Chytridiomycetes--and to assess the taxo...

BACKGROUND No attention has been paid so far to the preservation of fungal-treated lignocellulose for longer periods. In the present study, we treated wheat straw (WS) with the white-rot fungi Ceriporiopsis subvermispora and Lentinula edodes for 8 weeks and assessed changes in pH, chemical composition and in vitro gas production (IVGP) weekly. Fungal-treated WS was also stored for 64 days 'as i...

Background: Large amounts of fibre-rich organic waste material from public green and private gardens have to be treated environmentally friendly; however, this fibre-rich biomass has low biogas yields. This study investigated the presence of fungi in full-scale biogas plants as well as in laboratory reactors and elucidated the importance of fungi for the biogas process. Methods: The dominating ...