A subset of Wistar-Kyoto (WKY) rats that spontaneously develops biventricular hypertrophy (BVH) in response to increased cardiac output was evaluated for ventricular expression of the atrial natriuretic factor (ANF) gene. Normal WKY rats had low levels of left ventricular ANF mRNA and minimally detectable ANF transcripts in the right ventricle. In contrast, BVH rats showed a sixfold greater ANF...

BACKGROUND Studies were carried out to characterize several biochemical features of cultured AT-1 cells. METHODS AND RESULTS These cells were obtained from a transplantable atrial cardiomyocyte tumor lineage. Reverse transcriptase-polymerase chain reaction-based analyses demonstrated that the pattern of gene expression of cultured AT-1 cells was similar to that of adult atrial myocytes. AT-1 ...

This study examines the clearance and early hydrolysis of atrial natriuretic factor (ANF) in vivo. Radiolabeled ANF was cleared from the circulation of the rat with biphasic kinetics; the majority (90%) of ANF cleared with a t1/2 of 15 s, the remaining peptide was cleared with a t1/2 of 5 min. Microsequence analysis of ANF peptides recovered from the circulation of rats revealed five major degr...

The secretion of atrial natriuretic peptide by the heart is not simply the arterial-coronary sinus concentration difference times coronary blood flow, because only a small fraction of total coronary blood flow passes through the atria. We measured coronary sinus and arterial plasma atrial natriuretic factor (ANF) concentrations and blood flow to each part of the heart using the radioactive micr...

Atrial natriuretic factor receptor guanylate cyclase (ANF-RGC), was the first discovered member of the mammalian membrane guanylate cyclase family. The hallmark feature of the family is that a single protein contains both the site for recognition of the regulatory signal and the ability to transduce it into the production of the second messenger, cyclic GMP. For over two decades, the family has...

Specific, high affinity atrial natriuretic factor (ANF) binding sites were identified and localized by autoradiographic techniques in peripheral tissues of the guinea pig, rat, and human. In the guinea pig kidney, high concentrations of ANF binding sites were located in the glomerular apparatus, outer medulla, and small renal arteries. Other peripheral tissues containing ANF binding sites inclu...

The biologically active circulating form of atrial natriuretic factor (ANF) in the rat is the 28-amino-acid peptide ANF-(Ser-99-Tyr-126). Degradation of this peptide in vivo as well as in vitro, in whole blood, in plasma and by the isolated mesenteric artery was investigated. Studies in vivo in the rat demonstrated that the elimination and degradation of ANF was extremely fast: within 3 min mor...

It is now evident that atrial natriuretic factor (ANF) exerts neural effects in addition to vasorelaxant, depressor, and renal effects. The study by Ferrari et al examined the effects of intravenous ANF at doses within the pathophysiological range on arterial baroreceptor reflex control of heart rate and vascular resistance in conscious rats. Their results indicate that ANF markedly potentiated...

extracellular ca 2+ concentration ([ca2+]o) may be one of the factors affecting atrial natriuretic factor (anf) release. in the previous studies, there are opposite reports about the effect of [ca2+]o on anf secretion. therefore, the effect of [ca2+]o depletion on anf secretion and gene expression was studied in the present research. isolated right atria from rats were perfused for 6 and 2 hour...

Highly thermal conductivity materials with excellent electromagnetic interference shielding and Joule heating performances are ideal for management in the next generation of communication industry, artificial intelligence wearable electronics. In this work, silver nanowires (AgNWs) prepared using nitrate as source ethylene glycol solvent reducing agent, boron nitride (BN) is performed to prepar...