The cytotoxic cell granule secretory pathway is essential for host defense. This pathway is fundamentally a form of intracellular protein delivery where granule proteases (granzymes) from cytotoxic lymphocytes are thought to diffuse through barrel stave pores generated in the plasma membrane of the target cell by the pore forming protein perforin (PFN) and mediate apoptotic as well as additiona...

The present study was designed to investigate the in-vitro morphological assessment of apoptotic effect caused by nimbolide on the selected cancer cell lines (DU-145, PC-3, A-549) and normal cell lines (NIH3T3, CCD-18Co). The cells were grown in 6 well tissue culture plates after treatment with different concentrations of nimbolide and untreated control cells. Apoptotic and necrotic ce...

Zerumbone (ZER), a natural compound has been extracted from Zingiber zerumbet with known pharmacological activities. The aim was to determine the anti-human Burkitt’s lymphoma (Raji) cell effect of ZER. 3-(4,5-dimethylthiazol-2-yl)-2,5,-diphenyltetrazolium bromide (MTT) assay used cytotoxic while Annexin-V-fluorescein isothiocyanate/propidium iodide-PI flow cytometric assays apoptotic ZER on hu...

PURPOSE To develop a technology to image cell death in the eye of a live mouse and to apply that technology to characterize the role of apoptosis and necrosis in the evolution of endotoxin-induced uveitis (EIU), a standard model of intraocular inflammation. METHODS To induce EIU, 250 ng Escherichia coli 055:B5 lipopolysaccharide was injected into the vitreous body of BALB/c mice. At 0, 6, 10,...

Background: Dihydroartemisinin (DHA) is reported to be a potential anticancer agent, and the mechanisms underlying effects of DHA on diffuse large B cell lymphoma however are still obscure. This study aimed assess antitumor effect cells determine DHA-induced apoptosis. Methods: Here, Cell Counting Kit 8 assay was conducted proliferation. We performed Annexin V-FITC/propidium iodid...

The ox-LDL generated apoptotic bodies using THP-1 macrophage is a useful tool to study foam cell formation in atherosclerosis. However, the common problem cells negative control (i.e., absence of ox-LDL) undergo apoptosis. Therefore, type culture vessel was proposed be key factor contributing were differentiated into M1 macrophages 10 ng/μL PMA, 5 LPS, and 20 IFN-? while IL-4 IL-13 used differe...

amorphophallus campanulatus (roxb.) blume belonging to the family of araceae, is a perennial herb commonly known as elephant foot yam. its tuber has been traditionally used for the treatment of liver diseases, abdominal tumors, piles. the aim of the present study was to evaluate the dose-dependent cytotoxic and apoptosis inducing effects of the sub fractions of amorphophallus campanulatus tuber...

Objective: Silymarin is a herbal extract containing flavonolignans, and it has inhibitory effects against the growth of different cancer cell lines by inducing apoptosis. Toll-like receptors are suggested as a novel and attractive target to treat cancer. The current study aimed at examining the mechanism of silymarin-induced apoptosis in Ramos cells and investigating its effect...

BACKGROUND The serum and glucocorticoid inducible kinase SGK1, which was originally cloned from mammary tumor cells, is highly expressed in some but not all tumors. SGK1 confers survival to several tumor cells. Along those lines, the number of colonic tumors following chemical carcinogenesis was decreased in SGK1 knockout mice. Recently, a highly selective SGK inhibitor (EMD638683) has been dev...

BACKGROUND Death of smooth muscle cells in the atherosclerotic plaques makes the plaques more prone to rupture, which can initiate an acute ischemic event. The development of atherosclerosis includes the migration of immune cells e.g. monocytes/macrophages and T lymphocytes into the lesions. Immune cells can release antimicrobial peptides. One of these, human cathelicidin antimicrobial peptide ...