We investigated the presence of antibiotics in cryopreserved cardiovascular tissues and cryopreservation media, after tissue decontamination with antibiotic cocktails, and the impact of antibiotic residues on standard tissue bank microbiological analyses. Sixteen cardiovascular tissues were decontaminated with bank-prepared cocktails and cryopreserved by two different tissue banks according to ...

This study was based on the hypothesis that the occurrence of antimicrobial residues and antibiotic resistant bacteria in the sewage could be correlated with the structure and composition of the bacterial community and the antibiotic resistance loads of the final effluent. Raw and treated wastewater composite samples were collected from an urban treatment plant over 14 sampling dates. Samples w...

Thirty-one batchor continuous-flow milk pasteurizers used on commercial dairy and custom calf-feeding operations were surveyed. One sample of raw waste milk (RWM) immediately prior to onfarm pasteurization and one sample of pasteurized waste milk (PWM) immediately after on-farm pasteurization from daily waste milk pools were evaluated. The RWM and PWM samples were evaluated for nutrient composi...

Echinomycin producing Streptomyces cultures (X-53 or X-63) grown in the presence of quinazol-4-one-3-acetic acid yield an analogue in which one of the two quinoxaline-2-carboxyl residues is replaced by a quinazol-4-one-3acetyl moiety. This analogue has been designated quinazomycin. Cell-free extracts of culture X-63 .synthesize echinomycin in the presence of precursors, ATP, Mg2 + and mercaptoe...

New Delhi metallo-beta-lactamase (NDM-1) is an enzyme that makes bacteria resistant to a broad range of beta-lactam antibiotic drugs. This is because it can inactivate most beta-lactam antibiotic drugs by hydrolyzing them. For in-depth understanding of the hydrolysis mechanism, the three-dimensional structure of NDM-1 was developed. With such a structural frame, two enzyme-ligand complexes were...