PURPOSE Approximately 95% of patients who are diagnosed with Leber's hereditary optic neuropathy (LHON) have one of three mitochondrial point mutations responsible for the disease, G3460A, G11778A, and T14484C. The purpose of this study was to develop a novel multiplex real-time amplification-refractory mutation system (ARMS) PCR combined with high-resolution melt curves to identify the individ...

BACKGROUND We aimed to evaluate the feasibility of using the allele burden of Janus kinase 2 (JAK2) V617F as a criterion for discriminating 3 subtypes of Philadelphia chromosome-negative myeloproliferative neoplasm (Ph(-)MPN): polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF). METHODS We collected 70 peripheral blood (PB) and 81 bone marrow (BM) samples f...

abstract background: rhabdomyosarcoma is the most common soft tissue sarcoma among children which has two major subtypes: embryonal rhabdomyosarcoma (erms) and alveolar rhabdomyosarcoma (arms). distinction between these subtypes is mandatory to choose proper treatment and to determine prognosis. histopathologic study is the main method, but nowadays molecular studies like pcr are also used. the...

BACKGROUND Rh isoimmunization and hemolytic disease of the newborn still occur despite the availability of Rh immunoglobulin. For the prenatal investigation of sensitized RhD-negative pregnant women, determination of the zygosity of the RhD-positive father has important implications. The currently available molecular methods for RhD zygosity assessment, in general, are technically demanding and...

Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of mismatch bases. The optimal selection of primers can be achieved in an automated way using a program which evaluates candidate primers for a given...

INTRODUCTION The T to C transition at nucleotide 1565 of the human glycoprotein IIIa (ITGB3) gene represents a genetic polymorphism (PlA1/A2) that can influence both platelet activation and aggregation and that has been associated with many types of disease. Here, we present a newly designed multiplex tetra-primer amplification refractory mutation system - polymerase chain reaction (T-ARMS-PCR)...

Abstract Background: Rhabdomyosarcoma is the most common soft tissue sarcoma among children which has two major subtypes: embryonal rhabdomyosarcoma (ERMS) and alveolar rhabdomyosarcoma (ARMS). Distinction between these subtypes is mandatory to choose proper treatment and to determine prognosis. Histopathologic study is the main method, but nowadays molecular studies like PCR are also used...