Lysosomal enzyme release from PMN exposed to STZ was examined using PMN from normal and CGD donors. Normal PMN showed an increase of extracellular lysosomal enzyme activity and a marked reduction of totol (extra-plus intra-cellular) enzyme activity after phagocytosis. On the other hand, in PMN from CGD patients, such a reduction of total enzyme activity was not observed and much more enzyme tha...

Artificial cell-like communities participate in diverse modes of chemical interaction but exhibit minimal interfacing with their local environment. Here we develop an interactive microsystem based on the immobilization a population enzyme-active semipermeable proteinosomes within helical hydrogel filament to implement signal-induced movement. We attach large single-polynucleotide/peptide microc...

The inhibitory potential of purified α amylase inhibitors of sorghum on the digestive enzyme of Tribolium Castaneum, a devastating insect pest of economic importance have been studied. Qualitative and quantitative differences in α amylase of Tribolium Castaneum upon feeding on natural and artificial diets were evident. Natural diets were legumes like Black gram, Pigeon Pea, Red lentil, Cowpea a...

Engineering functional protein scaffolds capable of carrying out chemical catalysis is a major challenge in enzyme design. Starting from a noncatalytic protein scaffold, we recently generated a new RNA ligase by in vitro directed evolution. This artificial enzyme lost its original fold and adopted an entirely new structure with substantially enhanced conformational dynamics, demonstrating that ...

Artificial Compartmentalized Cascade Systems In article number 2300185, Wangsuk Oh, Dawoon Jeong, and Ji-Woong Park use a membrane with reticulated nanopores to confine one enzyme create compartment for another that allow cascade of biocatalytic reactions. This acts as both bioactive barrier means molecular transport between the internal external media, facilitating

1. A method is described for extraction and partial purification of mitochondrial l-3-glycerophosphate dehydrogenase from pig brain. 2. By the criteria that have so far been applied, the extraction and purification procedures do not modify the activity of the enzyme towards artificial electron acceptors. 3. The amounts of acid-liberatable flavine and iron in the preparation were measured. 4. A ...

In [1], Samoilov, Plyasunov, and Arkin provide an example of a chemical reaction whose full stochastic (Master Equation) model exhibits bistable behavior, but for which the deterministic (mean field) version has a unique steady state. The reaction that they provide consists of an enzymatic futile mechanism driven by a second reaction which induces " deterministic noise " on the concentration of...