SUMMARY The bone marrow of patients with acute lymphocytic leukemia, acute myelogenous leukemia, and metastatic cancer (normal marrow) was evaluated for L-asparagine dependence with the use of the in vitro test of Sobin and Kidd. All of the marrows including the normal ones showed L-asparagine dependence. There was a direct correlation between the concentration of L-asparaginase and depression ...

The proliferation and differentiation of rhizomes are crucial for the propagation Cymbidium species. We systematically assessed effects different concentrations 20 amino acids on C. goeringii rhizomes. Rhizome rates were significantly higher in media with 2.0 mmol·L −1 cysteine, 0.5 arginine, 0.2 asparagine, 1.0 proline, lysine compared those control. Additionally, tyrosine, aspartate beneficia...

L-asparaginases of agouti serum and Escherichia coli cause a profound lowering in the level of free asparagine in the blood of treated mice and also in the tissues. During treatment, normal tissues and resistant 6C3HED lymphomas survive unharmed with intracellular asparagine levels which are critically low for sensitive lymphomas. An explanation for this contrast between the two types of lympho...

The mammary gland is a system in which the components of the blood stream which are used by body tissues for protein synthesis can be readily investigated (Barry, 1956). Previous work has shown that the mammary gland takes the free essential amino acids from the blood stream to provide the essential amino acids of casein (Barry, 1952; Campbell & Work, 1952; Askonas, Campbell, Godin & Work, 1955...

The methyl-accepting chemotaxis protein, McpB, is the sole receptor mediating asparagine chemotaxis in Bacillus subtilis. In this study, we show that wild-type B. subtilis cells contain approximately 2,000 copies of McpB per cell, that these receptors are localized polarly, and that titration of only a few receptors is sufficient to generate a detectable behavioural response. In contrast to the...

The apoplast of developing soybean (Glycine max cv Hodgson) embryos and seed coats was analyzed for sucrose, amino acids, ureides, nitrate, and ammonia. The apoplast concentration of amino acids and nitrate peaked during the most rapid stage of seed filling and declined sharply as the seed attained its maximum dry weight. Amino acids and nitrate accounted for 80 to 95% of the total nitrogen, wi...

A study of the regulation of the synthesis of the enzyme glutamine synthase in Bacillus subtilis was initiated. An assay, based on the measurement of glutamo-hydroxamate, was used to characterize the enzyme in crude preparations and in toluene-treated cells. Determinations were made of the Michaelis constants for adenosine triphosphate, hydroxylamine, and glutamate (9 x 10(-3), 4 x 10(-3), and ...

X-linked sideroblastic anemia (XLSA) is due to deficient activity of erythroid-specific 5-aminolevulinate synthase (ALAS2). We report here a patient who developed sideroblastic anemia at the age of 81 years while undergoing hemodialysis. The diagnosis of sideroblastic anemia was established by the presence of ringed sideroblasts in the bone marrow, and treatment with oral pyridoxine completely ...