Carbofuran, a systemic N-methyl carbamate pesticide was orally administered with the doses of 0.4, 0.7, 1 and 1.3 mg/kg body weight/day to normal virgin female Swiss albino mice for 30 days. The vaginal smear and body weight of mice were recorded daily and mice were sacrificed on the 31st day. Estrous cycle was effected by showing a significant decrease in the number of estrous cycle and the du...

Phagocytic cells disposing of dying granulosa cells in the atretic ovarian follicles were morphologically studied in guinea pig ovaries at various stages of estrous cycle. Epon embedded semithin sections stained with toluidine blue were observed with a light microscope, and ultrathin sections were examined under a transmission electron microscope. Frozen sections were processed for acid phospha...

Ten, 4to 6-week-old BALB/c mice were randomly assigned to either control (non-vitrified, n = 5) or treatment (vitrified, n = 5) groups. Ovaries in the vitrified group were frozen sequentially by immersion into two vitrification solutions VS1: 10% ethylene glycol (EG) + 10% DMSO in holding medium (TCM-199 + 20% FBS) and VS2: 20% EG + 20% DMSO in holding medium. After thawing at 37 °C in 1.0 M su...

background: congenital toxoplasmosis is one cause of abortion. infection can disrupt ovarian cycles and because toxoplasmosis is an infectious disease may have a similar effect on the ovaries. the purpose of this study was to investigate the pathological changes in the ovaries due to toxoplasmosis.   methods: tachyzoites of toxoplasma gondii were harvested from peritoneal fluid of mice, experim...

The transcription factors GATA-4 and GATA-6, members of the GATA family, play an important role in ovarian cell proliferation, differentiation and apoptosis. In this study, the full-length coding sequences of goose GATA-4 and GATA-6 were cloned and characterized. GATA-4 and GATA-6 consist of 1236 and 1104 nucleotides encoding proteins with 411 and 367 amino acids, respectively. The deduced amin...

Endosulfan, a chlorinated cyclodiene insecticide, was administered orally at 1.5, 3, 6, and 9 mg/kg per day to normal hemicastrated virgin mice for 15 consecutive days to examine the effect on ovarian function. Sham-operated and hemicastrated control mice were administered a similar volume of olive oil. The vaginal smear and body weight of the mice were recorded daily and mice were sacrificed o...

A series of experiments was conducted to investigate the effect of basal feed level and increased feed intake during oestrus on ovulation rate, early embryo survival, and residual pituitary LH potency in the pig. All the gilts used were fed 1\m=.\8kg food/day from puberty to second heat. Gilts whose food intake was reduced from 1\m=.\8kg to 1\m=.\4 kg/day following second heat had more atretic ...

abstract increase in the use of nano zinc oxide in medicine, manufacturing cosmetic products and research, have raised the questions about their toxicity. zinc has direct role in growth, maturation and ovulation through influence on production of estradiol and progesterone. in this study the effects of nano zinc oxide on oogenesis of adult nmri mouse strain have been investigated. the adult fem...

BACKGROUND The physiological state of the dominant follicle is important as it may be linked to the competence of the oocyte within. The objective of this study was to analyze, by transcriptomic analysis, the changes occurring in granulosa cells from dominant follicles at different phases of follicular growth. METHODS Granulosa cells were collected from slaughterhouse dairy cattle follicles w...

Binding sites for IGF-I, insulin, and GH were localized by in situ binding of (125)I-labelled hormones to the different compartments of the sow ovary. Binding sites for IGF-I were detected in oocytes, granulosa and thecal cells of healthy and atretic follicles as well as in the antrum and the stroma. Competition of (125)I-labelled IGF-I with IGF-I, insulin and an analogue of IGF-I (Long R(3)IGF...