AIMS To evaluate the effect of topical autologous plasma on nerve morphology in patients with neurotrophic keratopathy (NK) using the confocal microscope. METHODS Eleven eyes of six patients with neurotrophic keratopathy were evaluated for this study. Corneal fluorescein staining was done, and corneal sensitivity measurements were done with the Cochet-Bonnet and modified Belmonte gas aesthesiom...

Twenty-two patients suspected of having either venous or arterial thrombi were studied with In-Ill-labeled autologous platelets. Whole-body scans were performed 3, 24, and 48 hr following i.v. injection. Twelve patients studied with saline-washed platelets had unsatisfactory 15-min recovery and biologic half-time. When the labeling was carried out in plasma, these values compared favorably with...

MicroRNAs (miRNAs) are small non-coding RNAs that regulate various biological processes. Cell-free miRNAs measured in blood plasma have emerged as specific and sensitive markers of physiological processes and disease. In this study, we investigated whether circulating miRNAs can serve as biomarkers for the detection of autologous blood transfusion, a major doping technique that is still undetec...

Strategies to reduce blood loss and transfusion of allogeneic blood products during surgical procedures are important in modern times. The most important and well-known autologous techniques are preoperative autologous predonation, hemodilution, perioperative red cell salvage, postoperative wound blood autotransfusion, and pharmacologic modulation of the hemostatic process. At present, new deve...

PURPOSE Optimized biocompatibility is a major requirement for alloplastic materials currently applied for stress urinary incontinence (SUI) and pelvic organ prolapse (POP) repair. In the preliminary studies the mesh modification by coating with autologous plasma resulted in the increased adherence score in vitro and improved biocompatibility in an animal model. The first use of plasma coated me...

periodontal regeneration in a one-wall intrabony defect is a challenging and complex phenomenon. the combination therapy of commercially available bone grafts with the innovative tissue engineering strategy, the platelet rich plasma, has emerged as a promising grafting modality for two and three walled intrabony osseous defects. the application of this combination approach was attempted in a mo...

INTRODUCTION: Platelet-rich plasma (PRP) belongs to one plasma division isolated by centrifugation of whole blood. From 1981 to 1991, a number of different growth factors containing platelets were reported one after another, such as platelet-derived growth factor (PDGF), transforming growth factor beta 1 (TGF-b1), transforming growth factor beta 2 (TGFb2), insulin-like growth factor (IGF), epid...

Cellular hypoxic preconditioning is being employed to obtain complex, yet physiological, secretomes rich is angiogenic factors. We previously proposed exposing peripheral blood cells (PBCs) to hypoxic stress stimulation, and demonstrated that controlled release of PBC-derived factor mixtures induces directional microvessel growth in vitro. Hypoxia therefore provides a useful tool for enhancing ...

INTRODUCTION: Intervertebral disc (IVD) degeneration is an important clinical problem that often contributes to low back pain and degenerative disc diseases. Degeneration of the IVD induces anulus tears and fissures, which can cause severe discogenic low back pain. Because the IVD has little potential to self-regenerate, treatment of degenerative disc disease is one of the most challenging clin...

With advances in basic science, the use of biological adjuncts is currently being explored to supplement orthopedic surgery. Platelet-rich plasma is a clinical concept of autologous intraoperative concentration of serum platelets to provide high levels of early critical growth factors for implantation into fracture, arthrodesis, or nonunion sites. The theoretical benefit of a biological adjunct...