Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis are members of the Bacillus cereus group of bacteria, demonstrating widely different phenotypes and pathological effects. B. anthracis causes the acute fatal disease anthrax and is a potential biological weapon due to its high toxicity. B. thuringiensis produces intracellular protein crystals toxic to a wide number of insect larvae...

Transformation of Bacillus anthracis with plasmid pUTE29-plcR-papR carrying the native Bacillus cereus plcR-papR gene cluster did not activate expression of B. anthracis hemolysin genes, even though these are expected to be responsive to activation by the global regulator PlcR. To further characterize the action of PlcR, we examined approximately 3,000 B. anthracis transformants containing pUTE...

W0 WO 01/21656 A2 W0 WO 01/82788 A2 3/2001 11/2001 Puziss et al (Journal of Bacteriology, v01. 85, 1963, p. 230-236).* Ramirez et al (Abstract of SIM Meeting, Nov. 1, 2001).* Skolnick et a1. (Trends in Biotechnology 18: 34-39, 2000).* Boslego et al (Vaccines and lmmunotherapy, 1991, Chapter 17).* Ellis (Vaccines, W.B. Saunders Company, Chapter 29, 1988, pp. 568-574).* Ahuja et al., “Rapid puri?...

Silence is a newly isolated siphophage that infects Bacillus megaterium, a soil bacterium that is used readily in research and commercial applications. A study of B. megaterium phage Silence will enhance our knowledge of the diversity of Bacillus phages. Here, we describe the complete genome sequence and annotated features of Silence.

Globally, anthrax outbreaks pose a serious threat to people, livestock, and wildlife. Furthermore, environmental change can exacerbate these outbreak dynamics by altering the host–pathogen relationship. However, little is known about how quantitative spatial of host movement may affect spread Bacillus anthracis, causative agent anthrax. Here, we use real-time observations high-resolution tracki...

Here we present vB_BanS-Tsamsa, a novel temperate phage isolated from Bacillus anthracis, the agent responsible for anthrax infections in wildlife, livestock and humans. Tsamsa phage is a giant siphovirus (order Caudovirales), featuring a long, flexible and non-contractile tail of 440 nm (not including baseplate structure) and an isometric head of 82 nm in diameter. We induced Tsamsa phage in s...

background and objectives : dna molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings. materials and methods : here a pcr-supported procedure is described that uses 10 primer pairs targeting chromosomal dna from the harmless vaccinal bacillus anthracis sterne 34f2 strain as template. a single pcr protocol is used to reproduce...

Variation among isolates of Bacillus anthracis was examined by using restriction fragmentation patterns and the PCR performed with arbitrary and sequence-specific oligonucleotide primers. The patterns were compared with the patterns generated from strains of closely related species belonging to the "Bacillus cereus group" of bacteria, including B. cereus, Bacillus thuringiensis, and Bacillus my...

1. Bacterial spores are highly resistant to the bactericidal action of the triphenylmethane dyes. Many Bacillus anthracis and Bacillus subtilis spores resist a saturated aqueous solution of gentian violet for 24 hours at 37 degrees C. They also resist exposure to the same dye solution for 10 minutes at 80 degrees C. 2. The selective bactericidal action of these dyes applies only to the vegetati...

Background: Antibacterial proteins are widely expressed in snake venoms. Previously, we have isolated two immunodominant proteins with molecular weights of 14 and 65kD from the snake venom of Naja naja oxiana (N. oxiana). It was demonstrated that they had potent inhibitory effects against gram-positive bacteria, S. aureus and B. subtilis but were less effective against gram-negative bacteria, s...