This book describes the fibrinolytic enzymes of microbial origin that are able to dissolve endogenous thrombi in vivo. The fibrinolytic enzyme streptokinase for example, is produced by b-hemolytic streptococci and exerts its enzyme action indirectly by activating plasminogen. On the other hand, staphylokinase is produced by Staphylococcus aureus by stoichiometric complexation with plasmin(ogen)...

Essential genes, those indispensable for the survival of an organism, play a key role in the emerging field, synthetic biology. Characterization of functions encoded by essential genes not only has important practical implications, such as in identifying antibiotic drug targets, but can also enhance our understanding of basic biology, such as functions needed to support cellular life. Enzymes a...

Knowledge of bacterial lipolytic enzymes is increasing at a rapid and exciting rate. To obtain an overview of this industrially very important class of enzymes and their characteristics, we have collected and classified the information available from protein and nucleotide databases. Here we propose an updated and extensive classification of bacterial esterases and lipases based mainly on a com...

This book describes the fibrinolytic enzymes of microbial origin that are able to dissolve endogenous thrombi in vivo. The fibrinolytic enzyme streptokinase for example, is produced by b-hemolytic streptococci and exerts its enzyme action indirectly by activating plasminogen. On the other hand, staphylokinase is produced by Staphylococcus aureus by stoichiometric complexation with plasmin(ogen)...

Ribonucleotide reductase (RNR) is a key enzyme that mediates the synthesis of deoxyribonucleotides, the DNA precursors, for DNA synthesis in every living cell. This enzyme converts ribonucleotides to deoxyribonucleotides, the building blocks for DNA replication, and repair. Clearly, RNR enzymes have contributed to the appearance of genetic material that exists today, being essential for the evo...

The goal of this study was to isolate and identify Staphylococcus aureus from clinical environmental sources. This also looked at the prevalence distribution different virulence enzymes among its isolates. For objective, 65 samples were collected dispersed 50 distinct 15 (soil, water, air) samples. results revealed that 20 bacterial isolates S. aureus. isolation rate 30.76% all samples, with 13...

for the enzyme in vivo unless an alkaline micro-environment was postulated or conditions were present in vivo which could potentiate inhibition by UMP at near-neutral pH (Yon, 19726). Substances such as deoxycholate which can bind to the hydrophobic site on the enzyme may provide such potentiation. Fig. 2 shows the effects of UMP and deoxycholate at pH values between 7 and 11. In the absence of...

Molybdoenzymes are widespread in eukaryotic and prokaryotic organisms where they play crucial functions in detoxification reactions in the metabolism of humans and bacteria, in nitrate assimilation in plants and in anaerobic respiration in bacteria. To be fully active, these enzymes require complex molybdenum-containing cofactors, which are inserted into the apoenzymes after folding. For almost...

This book describes the fibrinolytic enzymes of microbial origin that are able to dissolve endogenous thrombi in vivo. The fibrinolytic enzyme streptokinase for example, is produced by b-hemolytic streptococci and exerts its enzyme action indirectly by activating plasminogen. On the other hand, staphylokinase is produced by Staphylococcus aureus by stoichiometric complexation with plasmin(ogen)...