The aim of the present study was to assess the mutagenic potential of CVA1020 using the bacterial reverse mutation assay (Ames test) with bacteria and in vitro chromosomal aberration test with mammalian cells. In the reverse mutation test, Salmonella typhimurium TA 98, TA100, TA1535 and TA1537 and Escherichia coli [WP2 (uvrA)] were used with the dose range from 0.0015 to 0.16 μg/plate in tripli...

Previous research has shown that undenatured type II collagen is effective in the treatment of arthritis. The present study evaluated the broad-spectrum safety of UC-II by a variety of toxicological assays including acute oral, acute dermal, primary dermal irritation, and primary eye irritation toxicity. In addition, genotoxicity studies such as Ames bacterial reverse mutation assay and mouse l...

Magnesium stearate is widely used in the production of dietary supplement and pharmaceutical tablets, capsules and powders as well as many food products, including a variety of confectionery, spices and baking ingredients. Although considered to have a safe toxicity profile, there is no available information regarding its potential to induce genetic toxicity. To aid safety assessment efforts, m...

Forward mutation to 8-azaguanine resistance and reverse mutation to histidine prototrophy were measured in Salmonella typhimurium after treatment with 16 mutagens of both base-substitution and frameshift classes. The two approaches were found to be equisensitive for all 16 mutagens--i.e., induction of significant mutation occurred at similar concentrations in the forward mutation assay and in t...

India is an agrarian economy where use of pesticides has increased enormously to cater the food demands of increasing population. Imidacloprid is a relatively new systemic neonicotinoid insecticide with neurotoxic action as an alternative for commonly used organophosphorus insecticides in more than 100 countries. Imidacloprid is a nicotinic acetylcholine receptor agonist is an extensively used ...

This study is aimed at evaluating the potential of a biochip assay to sensitively detect KRAS mutation in DNA from non-small cell lung cancer (NSCLC) tissue samples. The assay covers 10 mutations in codons 12 and 13 of the KRAS gene, and is based on mutant-enriched PCR followed by reverse-hybridization of biotinylated amplification products to an array of sequence-specific probes immobilized on...

The focus of this research was to develop a better understanding of the pertinent physico-chemical properties of silver nanoparticles (AgNPs) that affect genotoxicity, specifically how cellular uptake influences a genotoxic cell response. The genotoxicity of AgNPs was assessed for three potential mechanisms: mutagenicity, clastogenicity and DNA strand-break-based DNA damage. Mutagenicity (rever...