Coronavirus disease (COVID-19) causes a serious threat to human health. Virus-like particles (VLPs) constitute promising platform in SARS-CoV-2 vaccine development. In this study, the E, M, and S genes were cloned into multiple cloning sites of new triple expression plasmid with one p10 promoter, two pPH promoters, three sites. The was transformed DH10 BacTMEscherichia coli competent cells obta...

The expression of recombinant protein for structure determination is one of the major challenges in pharmaceutical and academic research, since the number of potential drug targets has increased significantly in the last decade. Despite the fact that the baculovirus expression vector system is widely used for this purpose, the system is hampered by three very slow and tedious procedures, namely...

Although baculovirus has been used as a safe and convenient gene delivery vector in mammalian cells, baculovirus-mediated transgene expression is less effective in various mammalian cell lines. Identification of the negative regulators in host cells is necessary to improve baculovirus-based expression systems. Here, we performed high-throughput shRNA library screening, targeting 176 antiviral i...

The insect baculovirus expression vector system (BEVS) is useful for the production of biologically active recombinant proteins. However, the overexpression of foreign proteins in this system often results in misfolded proteins and the formation of protein aggregates. To overcome this limitation, we have developed a versatile baculovirus expression and secretion system using the Bombyx mori pro...

Vaccination is one of the most effective strategies to control epidemics. With deepening people’s awareness vaccination, there a high demand for vaccination. Hence, flexible, rapid, and cost-effective vaccine platform urgently needed. The baculovirus expression vector system (BEVS) has emerged as promising technology production due its safety, rapid production, flexible product design, scalabil...

Baculovirus expression system1s are a widely used tool in recombinant protein and biologics production. To enable the possibility of genome modifications unconstrained through low-throughput bespoke classical manipulation techniques, we set out to construct baculovirus vector (>130 kb dsDNA) built from modular, chemically synthesized DNA parts. We constructed synthetic version Autographa cal...

Foreign secretory pathway proteins are often produced in surprisingly low amounts in the baculovirus/insect cell expression system. One possible reason for this is that heterologous signal peptides might be inefficiently recognized by the insect cell protein translocation machinery. This idea was supported by a recent study showing that secretion of a plant protein in the baculovirus system was...