Sperm cryopreservation represents a useful tool in the management of reproduction in goat production. However, freezing and thawing produce physical and chemical stress on the sperm membrane that reduces their viability and fertilizing ability. In this study, firstly we evaluated the effects of reduced glutathione (GSH, 1 and 5mM) supplementation of the thawing extender on parameters of frozen-...

Background: Optimal thawing rate is critical for quality and fertilizability of frozen -thawed spermatozoa. Kinetic parameters are the most important factors which are affected by thawing rate so, that have close relation between kinetic parameters and fertility of the spermatozoa. Tris - egg yolk have been widely used for cryopreservation of the buffalo and other species semen. According to ou...

Computer-assisted sperm analyzers (CASA) have become the standard tool for evaluating sperm motility because they provide objective results for thousands of mammalian spermatozoa. Mammalian spermatozoa experience osmotic stress when the glycerol is added to the cells prior to freezing and removal from the cells after thawing. In order to minimize osmotic damage, cryoprotectants having lower mol...

BACKGROUND The objective of the study was to investigate the effects of in vitro zinc sulphate additive to semen extender on sperm parameters (progressive motility, viability, membrane integrity and DNA stability) after cryopreservation. MATERIALS AND METHODS In this Prospective longitudinal laboratory study, semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occa...

The present study was undertaken to examine the viability, motility and morphology of spermatozoa that were frozen-thawed at different temperature followed by the storage at 4oC until 10 hours under the condition of tropical area. The frozen semen derived from a Holstein-Friesian bull with the egg-yolkbase extender was thawed at 23oC or 37oC for 30 seconds or 15 seconds, respectively, and kept ...

Background: Since the deleterious effects of reactive oxygen species (ROS) during sperm cryopreservation can be overcome by antioxidants, post-thaw human sperm quality may perhaps profit from adding antioxidants to the extender media prior to cryopreservation. This study evaluated the effects of different concentrations of Trolox supplementation to cryo-protective media on post-thaw motility an...

The purpose of this study was to evaluate the probable effects of leptin addition in different levels to the semen extender on sperm quality (motility and motility parameters, viability, sperm membrane integrity, and DNA damage). Semen specimens were evaluated immediately after leptin addition, equilibration time and after thawing the frozen semen. Five healthy buffalo bulls (5 ejaculates from ...

AIM The present study was designed to evaluate the effect of supplementation of glutamine and replacement of Tris-egg yolk (TE) based buffer with defatted cow milk on the spermatozoa quality after equilibration and thawing. MATERIALS AND METHODS Semen was collected from five Bhadawari bulls biweekly, and a total of 30 ejaculates were taken. The semen ejaculates were pooled and divided into th...

The purpose of this study was to evaluate the probable effects of leptin addition in different levels to the semen extender on sperm quality (motility and motility parameters, viability, sperm membrane integrity, and DNA damage). Semen specimens were evaluated immediately after leptin addition, equilibration time and after thawing the frozen semen. Five healthy buffalo bulls (5 ejaculates from ...

In this study we evaluated the effects of semen extender supplementation with different concentrations of glutathione (GSH) on buffalo (Bubalus bubalis) bull sperm motility, plasma membrane integrity, viability and DNA integrity as well as in vivo fertility. Semen from three Nili-Ravi buffalo bulls was collected, and qualified semen ejaculates (n=18) were split into five aliquots for dilution (...