نتایج جستجو برای: basophil activation test
تعداد نتایج: 1222054 فیلتر نتایج به سال:
Diagnosis of diseases, especially qualification for treatment should be based on the methods properly optimized in order to minimize the risk of incorrect qualification to unnecessary treatments, or neglecting treatment wherever it is needed. Among possible alternatives for routine allergy diagnostic tests, hopeful mentions can be found of the basophil activation test (BAT), which replicates in...
BACKGROUND Subcutaneous Immunotherapy (SCIT) modifies the allergic response and relieves allergic symptoms. SCIT is the only and a very effective treatment for insect venom allergy. We hypothesized that basophil sensitivity, measured through the basophil activation test, would decrease during SCIT up dosing. Expression of CD203c was compared to CD63 as marker for basophil activation, using a Bl...
The basophil activation test (BAT) has proven to be a useful tool for the diagnosis of IgE-mediated food allergy and the evaluation of clinical tolerance in food allergic patients. Until now, successful oral desensitization to food allergen has been correlated with changes in cytokine production (IL-4 and INF-g) and allergenspecific IgE and IgG4 antibodies. We report a case of a 12-year-old mal...
Optimizing of the basophil activation test: Comparison of different basophil identification markers.
BACKGROUND Flowcytometric identification of basophils is a prerequisite for measuring activation of basophils with IgE-dependent or IgE-independent stimuli. Aim of this study was to compare different marker combinations in a simultaneous multicolor flowcytometric measurement. METHODS Ten patients with a grass pollen allergy and three controls were included in the study. Basophilic cells were ...
Methods Purified native peanut conglutin, containing Ara h2 and Ara h6, was chemically modified by reduction and alkylation (RA), without or with additional glutaraldehyde treatment (RAGA). Serum of 14 peanut allergic patients (DBPCFC-confirmed) was analyzed for peanut-specific antibodies by IgE immunoblot and IgE/IgG1/IgG4 ELISA. Allergen-induced basophil activation was analyzed by direct baso...
relationship to proportion of regulatory T cells. Methods: Forty-five patients with history of systemic symptoms of allergy to Hymenoptera venom were included. Basophil reactivity before the treatment and after one year of allergen immunotherapy (AIT) was measured by CD63 expression, CD203c marker used for basophil identification. Cells were stimulated by aqueous solution of allergen in concent...
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