The micropipet delivery-substrate collection (MD-SC) mode of scanning electrochemical microscopy (SECM) is demonstrated. This new mode is intended for the study and imaging of electrochemical as well as electrocatalytic reactions of neutral species that cannot be generated electrochemically. The spontaneous transfer of the analyte from an organic solvent across an interface between two immiscib...

We fabricated a thermostable quinoprotein aldose sugar dehydrogenase (tPQQ-ASD derived from a hyperthermophilic archaeon Pyrobaculum aerophilum) immobilized electrode. The electrode was prepared by immobilizing agarose gel mixed with the enzyme and carbon nanofiber (CNF) on a carbon paste (CP) electrode containing p-benzoquinone (BQ) as an electron mediator. The electrocatalytic response was ...

In this contribution we have studied the dynamics of light harvesting of ZnO nanoparticles (NPs) to a surface adsorbed sensitizing dye (SD) N719. By using the picosecond resolved Förster resonance energy transfer (FRET) technique we have explored that the excited ZnO NPs resonantly transfer visible optical radiation to the SD N719. The consequence of the energy transfer on the performance of th...

Benzene, a ubiquitous human carcinogen, forms DNA adducts through its metabolites such as p-benzoquinone (p-BQ) and hydroquinone (HQ). N(2)-(4-Hydroxyphenyl)-2'-deoxyguanosine (N(2)-4-HOPh-dG) is the principal adduct identified in vivo by (32)P-postlabeling in cells or animals treated with p-BQ or HQ. To study its effect on repair specificity and replication fidelity, we recently synthesized de...

Benzoquinones (BQ) have important functions in many biological processes. In alkaline environments, BQs can be hydroxylated at quinoid ring proton positions. Very little is known about the chemical reaction leading to these structural transformations as well as about the properties of the obtained hydroxyl benzoquinones. We analyzed the behavior of the naturally occurring 2,6-dimethoxy-1,4-benz...

The singlet excited states and doublet ionized/electron-attached states of o-benzoquinone (o-BQ) were studied by the symmetry adapted cluster (SAC)/SAC-configuration interaction (SAC-CI) method, and the corresponding spectral bands were assigned. The calculated transition energies reasonably reproduced the experimental spectra with the mean error of about 0.2 eV, except for the 1 1A1 states, wh...

According to WHO classification system, non-invasive urothelial carcinoma represents urothelial carcinoma in situ (CIS) and dysplasia. Dysplastic urothelium often progresses to CIS that further advances to urothelial carcinoma (UC). The strongest risk factor for UC is cigarette smoking. However, the pathogenesis of cigarette smoke (CS)-induced UC is poorly understood. Earlier we had shown that ...

BACKGROUND Cardiovascular disease (CVD) remains one of the major killers in modern society. One strong risk factor of CVD is cigarette smoking that causes myocardial injury and leads to the genesis of pathological cardiovascular events. However, the exact toxic component(s) of cigarette smoke (CS) and its molecular and cellular mechanisms for causing myocardial injury leading to heart damage an...

DNA methylation is a cell-type-specific epigenetic marker that essential for transcriptional regulation, silencing of repetitive and genomic imprinting. It also responsible the pathogenesis many diseases, including cancers. Herein, we present simple approach quantifying global in ovarian cancer patient plasma samples based on new class biopolymer nanobeads. Our utilises immune capture target el...

The magnetite/zinc oxide (Fe3O4/ZnO) nanocomposites were synthesized by the solid-state method using different ratios of zinc and iron oxide. structural, morphological, optical properties analysed X-ray diffraction (XRD), Fourier transform infrared spectra (FT-IR), Raman spectroscopy, transmission electron microscope (TEM), UV–Vis diffuse reflectance (UV–Vis/DRS) Photoluminescence (PL) spectrop...