The bacterial degradation of cholic acid under anaerobic conditions by Pseudomonas sp. N.C.I.B. 10590 was studied. The major unsaturated neutral compound was identified as 12 beta-hydroxyandrosta-4,6-diene-3,17-dione, and the major unsaturated acidic metabolite was identified as 12 alpha-hydroxy-3-oxochola-4,6-dien-24-oic acid. Eight minor unsaturated metabolites were isolated and evidence is g...

This paper describes the properties and partial purification from Pseudomonas testosteroni of enzymes concerned with the introduction of unsaturation into ring A of 3-ketosteroids. These enzymes promote the formation of the A1e4-diene-3-one structure from steroids bearing C-19 angular methyl groups and the formation of ring A phenols from lQ-nor-steroids. P. testosteroni is a strict aerobe whic...

From the Faculty of Engineering, Kyoto University, Kyotodaigaku-katsura, Saikyo-ku, Kyoto 615-8530, Japan, the Institute of Advanced Energy, Kyoto University, Gokasyo, Uji, Kyoto 611-0011, Japan, the Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan, and CREST, JST (Japan Science and Technology Agency), Gokasyo, Uji, Kyoto 611-00...

Ectopic expression of DEP domain containing 1 (DEPDC1) in lung adenocarcinomas is associated with poor prognosis, but its role and the underlying mechanism remain unknown. In this study, DEPDC1 expression in lung cancer cell lines was examined with Western blot assay, and DEPDC1-positive cell A549 was selected for further experiments. DEPDC1 inhibitor miR-130a was overexpressed in A549 cells, a...

mycobacterium smegmatis ptcc 1307 (cip 73.26) was used as a microbial agent to produce androsta-1,4-diene-3,17-dione (add) and androst-4-ene-3,17-dione, two useful precursors in the synthesis of steroid drugs. the side chain of cholesterol, as the substrate, was selectively cleaved in the presence of five enzyme inhibitors. an intermediate structure with intact side chain, cholest-4-ene-3-one, ...

Insulin-like growth factor-1 receptor (IGF-1R) is a growth factor receptor tyrosine kinase acting as a critical mediator of cell proliferation and survival. Novel 5-benzylidenethiazolidine-2,4-dione (5) and 5-(furan-2-ylmethylene)thiazolidine-2,4-dione (6) compounds were identified as potent and selective IGF-1R inhibitors via hierarchical virtual screening. Initial SAR and biological activity ...

GENERIC NAME PAGE Amodiaquine 614 Atovaquone 616 Chloroguanide 615 Chloroquine 613 Dapsone 615 Diloxanide 609 Eflornithine 610 Hydroxychloroquine 614 Iodoquinol 608 Mefloquine 616 Meglumine antimonate 611 Melarsoprol 610 GENERIC NAME PAGE Metronidazole 608 Nifurtimox 610 Paromomycin 609 Pentamidine 609 Primaquine 614 Pyrimethamine 614 Quinine 615 Sodium stibogluconate 611 Suramin 609 Trimethopr...

The localization and some characteristics of mouse adrenal C19-steroid 5fi-reductase were determined by the incubation of subcellular fractions of mouse adrenal tissue with [7a-3H]androst-4-ene-3,17-dione. This enzyme was present only in the soluble fraction and was NADPH-dependent, although a small activity in the presence ofNADH was also detected. The soluble fraction also contained 3 a-, 3,6...

An inferred ancient episode of heating and deformation on Tethys has been attributed to its passage through a 3:2 resonance with Dione (Chen, E.M.A., Nimmo, F. [2008]. Geophys. Res. Lett. 35, 19203). The satellites encounter, and are trapped into, the e-Dione resonance before reaching the e-Tethys resonance, limiting the degree to which Tethys is tidally heated. However, for an initial Dione ec...