The golden pompano snout (GPS) and head kidney (GPHK) cell lines have been proven to be meaningful tools for the study on pathogenic infections in vitro. In this study, we aimed select most stable reference genes from seven housekeeping (Actin, B2M, GAPDH, RPL13, EF1A, 18S UBCE) applied two of (GPS GPHK) under both normal physiological conditions stimulated lipopolysaccharide (LPS) or polyinosi...

Tea plant (Camellia sinensis) is an important woody economic crop used for processing leaf-type beverages. Numerous studies have shown that light a necessary environmental condition to control the growth and metabolism of C. sinensis. However, reference genes quantitative real time polymerase chain reaction (qRT-PCR) systematic analysis light-induced transcription mechanisms are still not avail...

The real-time quantitative reverse transcription PCR (RT-qPCR) is widely used for gene expression analysis, owing to its advantages of high specificity, sensitivity and repeatability. Suitable reference an absolute prerequisite accurate normalisation. In this study, three computational statistical methods before performing RT-qPCR, including geNorm, NormFinder BestKeeper was integrated stabilit...

The spotted scat (Scatophagus argus) is the most widely commercially cultured fish in eastern and southern Asia that possess potential economic value. However, up to date, there are no studies reported on selection of suitable reference genes this fish, which crucial for guaranteeing accurate reliable results RT-qPCR analyses. This study aimed at screening stable ovary pituitary different ovari...

Quinoa is a gluten-free food crop that contains all the essential amino acids and vitamins. The selection of proper housekeeping tissue-specific genes crucial prerequisite for gene expression analysis using common approach, real-time quantitative PCR (RT-qPCR). In this study, we identified 40 novel candidate by minimum transcript per million (TPM), coefficient variation (CV) maximum fold change...

Real time-qPCR is the most reliable method for evaluation of mRNA expression levels. However, to obtain accurate results, selection of suitable reference genes is necessary for normalizing the real-time qPCR data. The aim of this research was to validate the expression stability of three potential reference genes (ACTB, GAPDH and UXT) in milk somatic cells of Holstein dairy cattle under differe...

Parsley, one of the most important vegetables in the Apiaceae family, is widely used in the food, medicinal, and cosmetic industries. Recent studies on parsley mainly focus on its chemical composition, and further research involving the analysis of the plant's gene functions and expressions is required. qPCR is a powerful method for detecting very low quantities of target transcript levels and ...

The content stability of commonly used control genes is considered to vary significantly in different independent experimental systems, either in the expression of RNA expression or in the level of DNA content. The present study aimed to examine a panel of six common control genes, including β‑globin (HBB), telomerase (TERT), glyceraldehyde‑3‑phosphate dehydrogenase (GAPDH), albumin (ALB), β‑ac...