Previous studies have shown that Sindbis virus, an enveloped alphavirus of the togavirus group, activates the alternative complement pathway in the absence of detectable antiviral immunoglobulin. The present studies examined the role of the host-determined sialic acid content of Sindbis virus on activation of the alternative complement pathway. Purified Sindbis virus grown in baby hamster kidne...

Cells protect themselves from zinc toxicity by inducing proteins such as metallothionein (MT) that bind it tightly, by sequestering it in organelles, or by exporting it. In this study, the interplay between zinc binding by MT and its efflux by zinc transporter 1 (ZnT1) was examined genetically. Inactivation of the Znt1 gene in baby hamster kidney (BHK) cells that do not express their Mt genes r...

In previous reports, we have identified cell-surface glycopeptides from mouse cerebrum (BCSG) that inhibited protein synthesis and mitosis in several cell types. When baby hamster kidney (BHK)-21 cells were infected with vesicular stomatitis virus (a negative strand RNA virus), BCSG extensively inhibited viral protein synthesis. This inhibition was effective against both protein and glycoprotei...

Normal and transformed BHK-21 cells are heterogeneous in their capacity to support replication of polyoma virus. In situ hybridization has been used to demonstrate rare cells in which large numbers of polyoma genomes accumulate following infection by wild-type virus or induction of polyoma A gene mutants. Different transformed cell lines vary in the frequency of occurrence of these rare cells. ...

Mouse hepatitis virus (MHV) infection spreads from MHV-infected DBT cells, which express the MHV receptor CEACAM1 (MHVR), to BHK cells, which are devoid of the receptor, by intercellular membrane fusion (MHVR-independent fusion). This mode of infection is a property of wild-type (wt) JHMV cl-2 virus but is not seen in cultures infected with the mutant virus JHMV srr7. In this study, we show tha...

Theiler's murine encephalomyelitis virus is a neurotropic murine picornavirus which replicates permissively and causes a cytopathic effect in the BHK-21 cell line. We examined the interactions between the GDVII and DA strains of Theiler's virus and BHK-21 host cell proteins in a virus overlay assay. We observed binding of the virions to two proteins of approximately 60 kDa. These proteins were ...

of trypsin without EDTA. A monolayer of radioactively labelled BHK cells was incubated at 37°C for2min in 0.25 % trypsin; one half of the cells in suspension were harvested, washed and assayed for adhesion, and the other half of the cells were incubated for a further 15min in 0.25% trypsin before harvesting, washing and assaying for adhesion. Cells incubated in trypsin for only 2min did not sho...

in this study, the immunogenicity of a rabies vaccine that was used in one of the isfahan cattle farms is reported. this vaccine was produced by replication of the pasteur virus (pv) strain on bhk-21 monolayer cell culture. the virus suspension was inactivated by ß-propiolactone treatment and the harvested pool showed sterility and safety. thus, the obtained vaccine was tested on mice and a sat...

compared to other methods of vaccine production, (e.g. culture flask and bioreactor system), the roller culture method for producing rabies vaccine is simpler, and the equipment easier to install and maintain, and also in the event of contamination of one roller, the entire batch need not be discarded. this system provides a much larger surface area than the culture flask, and also ensures a mu...

We have used scanning electron microscopy and transmission electron microscopy to compare the initial adhesion of baby hamster kidney (BHK) cells and early passage human skin fibroblasts on glass, dried collagen, and hydrated collagen substrata. On glass or dried collagen substrata, BHK cell spreading required fibronectin and the cells were extensively flattened, with broad lamellipodia. In mar...