Plasmid profiles, genome restriction fragment polymorphisms, carbohydrate oxidation-fermentation reactions, methylumbelliferyl substrate hydrolysis patterns, antimicrobial susceptibilities, and results obtained with the Biolog GN biochemical substrate kit were used to type 19 common-source, but mixed-biotype, outbreak strains and one epidemiologically distinct strain of Pseudomonas pickettii. B...

BACKGROUND Helicobacter pylori colonizes almost half of the human population worldwide. H. pylori strains are genetically diverse, and the specific genotypes are associated with various clinical manifestations including gastric adenocarcinoma, peptic ulcer disease (PUD), and nonulcer dyspepsia (NUD). However, our current knowledge of the H. pylori metabolism is limited. To understand the metabo...

Routine procedures for isolating and characterizing microorganisms capable of degrading toxic chemicals are time consuming and labor intensive. The objective of this paper is to describe a new method for screening aerobic bacterial isolates and consortia that will rapidly determine metabolic capacity for various toxic chemicals and access the use of various substrates as inducers in the degrada...

Biolog Phenotype Microarray (PM) is a technology allowing simultaneous screening of the metabolic behaviour of bacteria under a large number of different conditions. Bacteria may often undergo several cycles of metabolic activity during a Biolog experiment. We introduce a novel algorithm to identify these metabolic cycles in PM experimental data, thus increasing the potential of PM technology i...

The effect of the pesticide Lindane on microbial populations was analyzed in soil with a history of contamination with various chemicals, including this pesticide. Soil microcosms were amended with 100 mg Lindane/kg soil and microbial populations were monitored for 70 days. Bacterial cell concentrations, metabolic versatility (whole community Biolog), and genetic diversity (16S rDNA/denaturing ...

Kocho and Bula are fermented product of Enset (Ensete ventricosum). It is the staple food for 20 million people in Ethiopia. The aim of study was to isolate, identify and characterize yeast species from fermented kocho and bulla by using Biolog Micro station. 300 Kocho samples were collected from Angacha District. 0.1ml of serially diluted samples were Streaked on yeast pepton dextrose agar and...

The widespread use and consumption of crude oil draws the public's attention to the fate of petroleum hydrocarbons in the environment, as they can permeate the soil environment in an uncontrollable manner. Contamination of soils with petroleum products, including diesel oil (DO), can cause changes in the microbiological soil properties. The effect of diesel oil on the functional diversity of fu...

E. Granda ([email protected]) and F. Valladares, Lab. Internacional de Cambio Global (LINC-Global), Depto de Biogeograf í a y Cambio Global, Museo Nacional de Ciencias Naturales, MNCN, CSIC, Serrano 115 Dpdo., ES-28006 Madrid, Spain. FV also at: Depto de Biolog í a y Geolog í a, ESCET, Univ. Rey Juan Carlos, Tulip á n s/n, ES-28933 M ó stoles, Spain. – C. Scoff oni and L. Sack, Dept of E...