Bioluminescence is the production and emission of light by a living organism. Bioluminescence imaging was developed over the last decade as a tool for studying biological processes in living small laboratory animals by molecular imaging. The bioluminescence-based optical imaging is highly sensitive, low-cost, and non-invasive, enabling the real-time analysis of disease processes within the cell...

Luciferase exhibits a broad range of emitting frequencies. Light emission from the bioluminescence of luciferase makes it an excellent tool for monitoring gene expression, thus the control of its bioluminescence color has great bio-analytical applications. Here I use an elastic network model to examine how the sequence distribution of luciferase is related to bioluminescence multicolor emission...

An expression vector for the luxAB genes, derived from Vibrio harveyi, was introduced into Nitrosomonas europaea. Although the recombinant strain produced bioluminescence due to the expression of the luxAB genes under normal growing conditions, the intensity of the light emission decreased immediately, in a time-and dose-dependent manner, with the addition of ammonia monooxygenase inhibitors, s...

Purpose We develop a quantitative bioluminescence assay for in vivo longitudinal monitoring of inflammation in animal models of uveitis. Methods Three models of experimental uveitis were induced in C57BL/6 albino mice: primed mycobacterial uveitis (PMU), endotoxin-induced uveitis (EIU), and experimental autoimmune uveitis (EAU). Intraperitoneal injection of luminol sodium salt, which emits li...

Bioluminescence, the creation and emission of light by organisms, affords insight into the lives of organisms doing it. Luminous living things are widespread and access diverse mechanisms to generate and control luminescence [1-5]. Among the least studied bioluminescent organisms are phylogenetically rare fungi-only 71 species, all within the ∼ 9,000 fungi of the temperate and tropical Agarical...

BACKGROUND Most methods for monitoring glucose level require an external energy source which may limit their application, particularly in vivo test. Bioluminescence technique offers an alternative way to provide emission light without external energy source by using bioluminescent proteins found from firefly or marine vertebrates and invertebrates. For quick and non-invasive detection of glucos...

Abstract The spontaneous emission spectrum from a driven three-level atom placed inside a double-band photonic crystal has been investigated. We use the model which assumes the upper levels of the atomic transition are coupled via a classical driving field. The transition from one of the upper levels to lower level couples to the modes of the modified reservoir, and the transition from the oth...

Bioluminescence is a common ecological trait among many marine organisms, including three shark families: Etmopteridae, Dalatiidae, and Somniosidae. The kitefin shark, Dalatias licha (Bonnaterre, 1788), from the Dalatiidae family largest known luminous vertebrate. This study compares light organ ultrastructure of D. with that Etmopterus spinax, type species to gain deeper understanding emission...

The emission of blue-green bioluminescence ({lambda}max = 470 nm) was observed from sucker-like structures arranged along the length of the arms of the cirrate octopod Stauroteuthis syrtensis. Individual photophores either glowed dimly and continuously or flashed on and off more brightly with a period of 1-2 seconds. Examination of the anatomy and ultrastructure of the photophores confirmed tha...

Fluorescence and bioluminescence are widely used to study biological systems from molecular to whole organism level. However, their broadband emission is often a bottleneck for sensitive spectral measurements and multiplexing. To overcome the limitation, the emitters can be coupled with optical cavity modes to generate narrowband spectral features. Here we demonstrate several types of emitter-r...