Abstract In vivo phage display is widely used for identification of organ- or disease-specific homing peptides. However, the current in biopanning approaches fail to assess biodistribution specific peptide phages across tissues during screen, thus necessitating laborious and time-consuming post-screening validation studies on individual phages. Here, we adopted bioinformatics tools RNA sequenci...

A phage repertoire was constructed using antibody genes from the bone marrow and the spleen of a rabbit immunized with Cry1Ab toxin. Biopanning against either the Cry1Ab toxin or a domain II loop 3 synthetic peptide resulted in the identification of monoclonal antibodies in scFv format. They inhibited binding and toxicity of Cry1Ab toxin against Manduca sexta. Toxin overlay assays, using the sc...

Domain III of the dengue virus envelope protein (EDIII, aa295-395) has an immunoglobulin fold and is the proposed receptor-binding domain of the virus. Previous studies have shown that monoclonal antibodies against EDIII can be neutralizing and have therapeutic potential. Here, cloned Fab-phage libraries of human and mouse origin were screened for DENV specific antibodies. Firstly, bacterially ...

The multidimensional nature of dengue virus (DENV) infections, which can be caused by four distinct serotypes of the virus, complicates the sensitivity of assays designed for the diagnosis of infection. Different viral markers can be optimally detected at different stages of infection. Of particular clinical importance is the early identification of infection, which is pivotal for disease manag...

To elucidate unknown mammalian peroxisomal enzymes and functions, we subjected M13 phage expressing fusions between the gene encoding protein VI and a rat liver cDNA library to an immunoaffinity selection process in vitro (biopanning) with the use of antibodies raised against peroxisomal subfractions. In an initial series of biopanning experiments, four different cDNA clones were obtained. Thes...

PURPOSE Phage display technology can be used to identify peptide sequences that bind rapidly and specifically to tumors responding to sunitinib therapy. These peptides may help to address problems with current methods of assessing tumor response to therapy that can be slow and have limited usage. EXPERIMENTAL DESIGN The peptide of interest was isolated after four rounds of biopanning in MDA-M...