BACKGROUND Polyhydroxyalkanoates (PHA) are synthesized by many bacteria in the cytoplasm as storage compounds for energy and carbon. The key enzymes for PHA biosynthesis are PHA polymerases, which catalyze the covalent linkage of 3-hydroxyacyl coenzymeA thioesters by transesterification with concomitant release of CoA. Pseudomonas putida GPo1 and many other Pseudomonas species contain two diffe...

This study investigated the apparent genetic redundancy in the biosynthesis of polyhydroxyalkanoates (PHAs) in the Rhodospirillum rubrum genome revealed by the occurrence of three homologous PHA polymerase genes (phaC1, phaC2, and phaC3). In vitro biochemical assays established that each gene product encodes PHA polymerase. A series of single, double, and triple phaC deletion mutants were chara...

Polyhydroxyalkanoates (PHAs) are a family of biopolyesters accumulated by a variety of microorganisms as carbon and energy storage under starvation conditions. We focused on marine purple non-sulfur photosynthetic bacteria as host microorganisms for PHA production and developed a method for their isolation from natural seawater. To identify novel PHA-producing marine purple non-sulfur photosynt...

Polyhydroxyalkanoates (PHAs) are a group of carbon andenergy storage compounds that are accumulated during suboptimal growth by many bacteria, and intracellularly deposited in the form of inclusion bodies. Accumulation of PHAs is thought to be used by bacteria to increase survival and stress tolerance in changing environments, and in competitive settings where carbon and energy sources may be l...

Objective: Analyze the production of polyhydroxyalkanoates (PHA) by Bacillus subtilis sp., in submerged culture using pear residues as a carbon source.
 Design/methodology/approach: The consisted residue flour concentrations 5 and 15 % for 72 h. Reducing sugars, biomass, pH, protein, pHA extraction quantification were analyzed during submerging cultivation subtilis
 Results: results s...

Polyhydroxyalkanoates (PHAs) are a family of biopolyesters synthesized by many types of bacteria as carbon and energy reserve materials. PHAs combine properties of thermal processibility, biodegradability, biocompatibility and sustainability. They have attracted attention from fermentation, materials and biomedical industries. Recent environmental concerns such as CO(2) emissions and plastic po...

Polyhydroxyalkanoates (PHA) are biodegradable and biocompatible green thermoplastics, synthesized by wide variety of bacteria as an intracellular carbon and energy storage intermediate. They are used as an alternative to nonrenewable petroleum derived plastics. The current interest in these biopolyesters is stimulated by the search for cost-effective capitalized production. This paper attempts ...

Polyhydroxyalkanoates (PHAs) is a group of biopolymers that are extensively researched for such purpose due to the biocompatibility with mammal tissue and similar properties with conventional plastic. However, commercialization of PHA is impended by its high total production cost, which half of it are from the cost of pure carbon source feedstock. Thus, cheap and sustainable feedstocks are pref...

Polyhydroxyalkanoate commercial manufacture is hampered by high production costs (PHAs). Up to 50% of the total process expenses might be attributed medium used for cultivation. With this research, researchers hoped determine if acidogenic fermentation food waste may yield volatile fatty acids as low-cost carbon sources bacterial growth essential make polyhydroxyalkanoates The bacterium Pseudom...

Polyhydroxyalkanoates (PHA) producers have been found in a variety of ecological niche’s that are naturally or unintentionally exposed to high organic matter growth limiting substances such as dairy wastes, hydrocarbon contaminated sites, pulp and paper mill agricultural activated sludges treatment plants, rhizosphere, industrial effluents. Few them also create extracellular byproducts rhamnoli...