Alternaria sp. MG1, an endophytic fungus previously isolated from Merlot grape, produces resveratrol from glucose, showing similar metabolic flux to the phenylpropanoid biosynthesis pathway, currently found solely in plants. In order to identify the resveratrol biosynthesis pathway in this strain at the gene level, de novo transcriptome sequencing was conducted using Illumina paired-end sequenc...

abstract benzylisoquinoline alkaloids are a diverse group of nitrogenous compounds which is found in about 20% of plant species. isolation of effective genes involved in morphine biosynthesis of opium poppy is very important in the production of specific which can be achieved using metabolic engineering techniques. in this biosynthesis pathway, the key enzyme sat is involved in the conversion o...

Studies of de novo cytokinin biosynthesis in isopentenyltransferase (ipt)-transformed Arabidopsis thaliana, involving in vivo deuterium labeling and mass spectrometry, showed that the biosynthetic rate of zeatinriboside-5'-monophosphate was around 66-fold higher than that of isopentenyladenosine-5'-monophosphate (iPMP), the proposed primary product of the Agrobacterium ipt. Double tracer analys...

The red root of Salvia miltiorrhiza Bunge, a famous traditional Chinese medicine (TCM), was caused by tanshinone in epidermis cells. In order to study the biological function ncRNAs synthesis, expression patterns mRNA and were comprehensively analyzed (high content) white (low tissues derived from same plant. A total 731 differentially expressed genes (DEGs) mainly enriched primary metabolic pa...

5-Aminoimidazole-4-carboxamide-1-beta-d-ribofuranoside (AICAr), a commonly used indirect activator of AMP-activated protein kinase (AMPK), inhibits phosphatidylcholine (PC) biosynthesis in freshly isolated hepatocytes. In all nucleated mammalian cells, PC is synthesized from choline via the Kennedy (CDP-choline) pathway. The purpose of our study was to provide direct evidence that AMPK regulate...

BACKGROUND Steviol glycoside biosynthesis pathway has emerged as bifurcation from ent-kaurenoic acid, substrate of methyl erythritol phosphate pathway that also leads to gibberellin biosynthesis. However, the genetic regulation of steviol glycoside biosynthesis has not been studied. So, in present study RNA interference (RNAi) based Agrobacterium mediated transient gene silencing (AMTS) approac...

Soybean (Glycine max) is one of the most important oilseed crops. However, regulatory mechanism that governs process oil accumulation in soybean remains poorly understood. In this study, GmZF392, a tandem CCCH zinc finger (TZF) protein which was identified our previous RNA-seq analysis seed-preferred transcription factors, found to function as positive regulator lipid production. GmZF392 promot...

Abstract ?-Cadinol is a sesquiterpene that widely used in perfume, fine chemicals and medicines industry. In this study, we established biosynthetic pathway for the first time engineered Escherichia coli production of ?-cadinol from simple carbon sources. Subsequently, further improved to 35.9 ± 4.3 mg/L by optimizing overproduction rate-limiting enzyme IdI. Finally, titer was increased 133.5 1...

The title compound, C(26)H(39)NO(7), which was isolated from Delphinium anthriscifolium var. majus, has a lycoctonine carbon skeleton containing four six-membered rings (A, B, D and E) and three five-membered rings (C, F and G). Rings A, B and E adopt chair conformation, while ring D adopts a boat conformation. Rings C and F adopt envelope conformations.

Bdellovibrio bacteriovorus 109J, a predatory bacterium with potential as a bacterial control agent, can exist in several lifestyles that differ both in predatory capacity and color. We determined that levels of ubiquinone-8 contribute to the distinctive but variable yellow color of different types of Bdellovibrio cells. Steady-state ubiquinone-8 concentrations did not differ markedly between co...