نتایج جستجو برای: biotin

تعداد نتایج: 7604  

Journal: :Genome research 2002
Dmitry A Rodionov Andrei A Mironov Mikhail S Gelfand

Biotin is a necessary cofactor of numerous biotin-dependent carboxylases in a variety of microorganisms. The strict control of biotin biosynthesis in Escherichia coli is mediated by the bifunctional BirA protein, which acts both as a biotin-protein ligase and as a transcriptional repressor of the biotin operon. Little is known about regulation of biotin biosynthesis in other bacteria. Using com...

Journal: :Canadian journal of physiology and pharmacology 2015
Krishnamurti Dakshinamurti Rushita A Bagchi Bernard Abrenica Michael P Czubryt

Biotin is a B vitamin involved in multiple metabolic pathways. In humans, biotin deficiency is relatively rare but can cause dermatitis, alopecia, and perosis. Low biotin levels occur in individuals with type-2 diabetes, and supplementation with biotin plus chromium may improve blood sugar control. The acute effect on pancreatic gene expression of biotin repletion following chronic deficiency i...

2007
Danielle Russell Joshua D. Levin

SureLINK Chromophoric Biotin Labeling Kits allow for the easy labeling of proteins with biotin as well as an accurate, precise, and inexpensive method of quantitating biotin in biotin-protein conjugates. Accurate measurement of this parameter is critical for the efficient design of reproducible immunoassays. The basis of the quantitation method is a biotinylation reagent that contains a chromop...

Journal: :Analytical biochemistry 2002
James D Hirsch Leila Eslamizar Brian J Filanoski Nabi Malekzadeh Rosaria P Haugland Joseph M Beechem Richard P Haugland

The high-affinity binding of biotin to avidin, streptavidin, and related proteins has been exploited for decades. However, a disadvantage of the biotin/biotin-binding protein interaction is that it is essentially irreversible under physiological conditions. Desthiobiotin is a biotin analogue that binds less tightly to biotin-binding proteins and is easily displaced by biotin. We synthesized an ...

Journal: :The British journal of nutrition 1980
C C Whitehead

1. The effect of biotin supplementation of low-biotin practical diets during rearing and laying stages on performance was studied in White Leghorn-type laying hens. 2. Biotin supplementation during rearing did not have any apparent effect on the condition of the bird at point-of-lay or on subsequent laying performance. 3. Biotin supplementation during lay did not have any beneficial effect upon...

Journal: :PLoS ONE 2008
Sudha Purushothaman Garima Gupta Richa Srivastava Vasanthakumar Ganga Ramu Avadhesha Surolia

BACKGROUND Fatty acids are indispensable constituents of mycolic acids that impart toughness & permeability barrier to the cell envelope of M. tuberculosis. Biotin is an essential co-factor for acetyl-CoA carboxylase (ACC) the enzyme involved in the synthesis of malonyl-CoA, a committed precursor, needed for fatty acid synthesis. Biotin carboxyl carrier protein (BCCP) provides the co-factor for...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1995
T Sano C R Cantor

In natural streptavidin, tryptophan 120 of each subunit makes contacts with the biotin bound by an adjacent subunit through the dimer-dimer interface. To understand quantitatively the role of tryptophan 120 and its intersubunit communication in the properties of streptavidin, a streptavidin mutant in which tryptophan 120 is converted to phenylalanine was produced and characterized. The streptav...

Journal: :The Journal of clinical endocrinology and metabolism 2016
Marianne S Elston Shekhar Sehgal Stephen Du Toit Tania Yarndley John V Conaglen

CONTEXT Biotin (vitamin B7) is an essential co-factor for four carboxylases involved in fatty acid metabolism, leucine degradation, and gluconeogenesis. The recommended daily intake (RDI) of biotin is approximately 30 μg per day. Low-moderate dose biotin is a common component of multivitamin preparations, and high-dose biotin (10 000 times RDI) has been reported to improve clinical outcomes and...

Journal: :Yeast 2003
Jürgen Stolz

The fission yeast Schizosaccharomyces pombe is auxotrophic for biotin (vitamin H) and growth depends on biotin uptake over the plasma membrane. Here a biotin transport mutant of Saccharomyces cerevisiae is used to identify the vht1(+) gene encoding the Schizosaccharomyces pombe plasma membrane transport protein for biotin. SpVht1p belongs to the family of allantoate transporters and has only li...

Journal: :Annals of the New York Academy of Sciences 1985
K Dakshinamurti L Chalifour R P Bhullar

The requirement of biotin in the culture medium has been established for HeLa cells, human fibroblasts, baby hamster kidney cells (BHK) as well as polyoma-transformed BHK cells. Growth, viability, biotin content and the activities of biotin-dependent enzymes were used as criteria. Cells in culture appear to bind and internalized avidin as well as the avidin-biotin complex. Avidin seems to mimic...

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