The genus Pestivirus of the family Flaviviridae consists of four recognized species: Bovine viral diarrhoea virus 1 (BVDV-1), Bovine viral diarrhoea virus 2 (BVDV-2), Classical swine fever virus (CSFV) and Border disease virus (BDV). Recently, atypical pestiviruses ('HoBi'-like pestiviruses) were identified in batches of contaminated foetal calf serum and in naturally infected cattle with and w...

A wave of abortions, stillbirths and deformities in sheep occurred at the Al-Ahsa oasis in the eastern region of Saudi Arabia in the second half of 1999. The abortions were recorded in August and September and stillbirths and deformities in neonates were observed in October. Adult sheep were clinically normal. A virus was isolated in chicken embryos, adapted to Vero cell culture and further ide...

Ruminant pestivirus infections of pigs have a worldwide distribution. The prevalence is varied and depends mainly on (i) contact with cattle, (ii) age of pigs and (iii) degree of homology of virus strains used for serology, with field strains of bovine virus diarrhoea virus (BVDV) infecting pigs. Emphasis should be laid on sources of BVDV other than cattle, e.g. contaminated vaccines and fetal ...

Enzootic bovine leukosis (EBL) is known as bovine lymphosarcoma and normally affects the old cattle. EBL is caused by bovine leukemia virus (BLV), which is generally spread all around the world. This virus is transmitted via bovine blood products within and between cattle herds. Glycoprotein GP51 in the blood is responsible for cattle immune responses to BLV. This virus has been previously dete...

Twelve batches of commercially available foetal bovine sera were tested for the presence of bovine viral diarrhoea virus (BVDV) and BDVD antibodies. The antibodies were found in three batches coming from two suppliers. Fragments of BVDV genome were present in five of these batches when tested by RT-PCR. All of them were type 1 BVDV according to a direct sequencing of PCR products. However, the ...

The current situation of bovine virus diarrhoea-mucosal disease (BVD-MD) virus infections is briefly reviewed, with special reference to problems arising from fetal infection in the Hokkaido district, a northern island of Japan. The results of some investigations on BVD-MD virus carried out in Japan are also described. Although bovine congenital anomalies caused by BVD-MD virus and mucosal dise...

Abstract Background and Aims: To identify and isolation of viruses a susceptible cell is need which can be primary or line. The use of primary cells is laborious, time consuming and expensive, while the use of cell lines is much easier and faster. IRKHBK cell line was originated from primary cells of bovine kidney by continuous passage. The aim of this study was to introduce the IRKHBK cell ...

The genus Pestivirus comprises four recognised species: Bovine viral diarrhoea virus 1 (BVDV-1), Bovine viral diarrhoea virus 2 (BVDV-2), Border disease virus (BDV), and Classical swine fever virus (CSFV); and a tentative species, Pestivirus of giraffe. Recently, additional pestiviruses have been identified and presumed as novel subgroups/species based on single gene analyses that reveal differ...

bovine ephemeral fever (bef) is an acute epidemic disease in cattle and water buffalo, spanning tropical and subtropical zones of asia, australia, and africa. in recent years, bef has been distributed in many provinces of iran and caused economic losses. in this study the sequence encoding the g1 epitope of bovine ephemeral fever virus (befv) g glycoprotein was amplified by pcr, ligated into th...

the objective of this study was to compare an indirect elisa, based on a purified 60 kda envelope glycoprotein (gp51su), with a pourquire indirect elisa for the detection of antibodies to the bovine leukemia virus. for conducting this research, 340 serum samples were collected from two different breeds of cows (sarabi and holestin) in different herds. commercial elisa revealed positive results ...