A candidate gene analysis of the microphthalmia-associated transcription factor (MITF) gene was used in an attempt to identify the genetic basis for a white-spotted coat color phenotype in the Asian swamp buffalo (Bubalus bubalis carabanensis). Ninety-three buffaloes-32 solid, 38 spotted and 23 white individuals-were Sanger-sequenced for all MITF exons as well as highly conserved intronic and f...

ABSTRACT: This study presented relevant aspects about the Amazonian environment and how it impacts thermal comfort of domestic buffaloes (Bubalus bubalis) raised in Eastern Amazon. Furthermore, strategies for monitoring mitigating animal heat stress are presented, based on research results with species. Although considered adaptable animals, exposure to intense solar radiation causes discomfort...

Objective: Diacylglycerol acyltransferase 1 is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The objective of this project is to check out the polymorphism at the exon 8 region of DGAT1 gene using PCR-SSCP technique in Iranian water buffaloes (Bubalus bubalis). Recent activities have shown that a significant association between lysine at amino acid positi...

The Asian buffalo or the Water buffalo is classified in the genus Bubalus, species bubalis. Taming occurred at recent times (5,000 years ago) compared to taming of Bos Taurus and Bos Indicus (10,000 years ago). Asian buffalo includes two subspecies named also types: the River and the Swamp, the morphology and the purposes of which are different as well as the genetics. The River buffalo has 50 ...

OBJECTIVE The present study was conducted to investigate the seroprevalence of Toxoplasma gondii infection in water buffaloes. METHODS The research was conducted on 131 water buffaloes from Samsun and Afyon provinces. Serum samples of buffaloes were tested for toxoplasmosis with the Sabin Feldman Dye Test (SFDT), using live tachyzoites and methylene-blue dye. RESULTS 87.79% of buffaloes had...

Gene expression studies require appropriate normalization methods for proper evaluation of reference genes. To date, not many studies have been reported on the identification of suitable reference genes in buffaloes. The present study was undertaken to determine the panel of suitable reference genes in heat-stressed buffalo mammary epithelial cells (MECs). Briefly, MEC culture from buffalo mamm...

The water buffalo (Bubalus bubalis) is an important dairy animal on the Indian subcontinent and in Southeast Asian countries. The diversity and differentiation among 12 populations or breeds of buffalo were studied. Data were generated and analyzed from 527 animals belonging to 10 recognized breeds and 2 additional populations of Indian buffalo by using 22 microsatellite loci. Relationships amo...

The water buffalo (Bubalus bubalis) is considered an efficient converter of poor quality forages into high quality milk and meat. This species is ubiquitous, with prevalence though in Asian and Mediterranean countries. From a genetic standpoint, the species is characterized by two main subspecies: river and swamp type. The former to be found predominantly in Mediterranean countries, whereas the...