Rabbits immunized with mucopeptide derived from cell wall debris of Streptococcus agalactiae by use of the formamide extraction technique developed specific antibodies for bovine heart, skeletal muscle, lymph nodes, and blood buffy coat extracts.

Background: Besides charging the patients with outstanding costs, tuberculosis (TB) causes high mortality and morbidity in a country. We studied the sensitivity, specificity, positive and negative predictive values as well as the efficiency of buffy coat smear test in patients who were pulmonary TB suspects. This research was conducted at Massih Daneshvari Hospital, National Research Institute ...

The polymerase chain reaction (PCR) has been used to detect Legionella DNA in respiratory tract, serum, and urine samples from patients with pneumonia. In addition, a preliminary study using a guinea pig model suggested that testing of peripheral leukocytes by PCR may be more sensitive than testing of other samples. We used PCR to detect Legionella DNA in serial peripheral leukocyte (buffy coat...

The morphological identification of Trypanosoma cruzi is currently considered to have a high specificity, but its sensitivity, which depends on the volume of the sample examined, is rather low. Trypanosome developmental stages suspended in blood, reduviid feces, and culture media are routinely searched for by means of fresh film examination (about 2 microL). High speed centrifugation of blood s...

Patients with severe aplastic anemia were conditioned with cyclophosphamide (200 mg/kg) and given marrow grafts from HLA-identical family members. Among 233 patients transplanted, 225 survived greater than or equal to 14 days and could be evaluated for engraftment. Forty-four of the 225 rejected their graft; 33 of these died and 11 survive. One hundred eighty-one patients had sustained engraftm...

PURPOSE AND EXPERIMENTAL DESIGN Using real-time quantitative methylation-specific PCR (RTQ-MSP), we quantified methylated p16INK4a sequences and determined the fractional concentrations of circulating tumor DNA in plasma, serum, and peripheral blood cells collected preoperatively, intraoperatively, and postoperatively from 49 patients with hepatocellular carcinoma (HCC). RESULTS RTQ-MSP was s...

By P. EFRATI AND L. ROZENSZAJN E XA\IINING peripheral blood for L.E. cells, we encountered cells which are not usually found in blood smears. \\Te decided therefore to study the mlior )hologv of ieukccvte concentrates of healthy adults. II. Klima of Vienna has since l949 used the above method to investigate 1)eril)lleral blood morphology in various diseases. Although his material was vast, ve c...

Bovine viral diarrhea virus (BVDV) is one of the most important viral pathogens of cattle worldwide. The aim of present study was to determine the molecular characterization and phylogenetic analysis of BVDV infection in dairy herds of Fars province, Iran. For initial screening, a total of 400 blood samples were collected from 12 industrial dairy herds with previous history of diarrhea, abortio...