background: : permanent slide preparation of nematodes especially small ones is time consuming, difficult and they become scarious margins. regarding this prob­lem, a modified double glass mounting method was developed and com­pared with classic method. methods: a total of 209 nematode samples from human and animal origin were fixed and stained with formaldehyde alcohol azocarmine lactophenol (...

WEBSTER, J. M. 1973. Manipulation of environment to facilitate use of nematodes in biocontrol of insects. Experimental Parasitology 33, 197-206. The preand postparasitic phases of entomophilic nematodes are exposed to different microenvironments which should be changed in order to facilitate the use of nematodes as biological control agents of insects. The mixing of “thick water” with nematodes...

Anhydrobiotic nematodes were fixed and extracted from dry Mojave desert soils with hot and cold fixatives (5% formalin and 4% gluteraldehyde). Morphologically, extracted nematodes were tightly coiled and shrunken in size. Various concentrations of KCl, ethylene glycol, and sucrose solutions were compared for their effectiveness in extracting viable nematodes still in the anhydrobiotic state. Ap...

Background: The social amoeba Dictyostelium discoideum is a soil-dwelling microbe, which lives most of its life cycle in the vegetative stage as a predator of bacteria and as prey for nematodes. When bacteria are sparse, amoebae aggregate into a multicellular fruiting body. Some clones of D. discoideum have agriculture (Brock et al., 2011). They carry bacteria through the social stage, eat them...

The infectivity of four species of entomopathogenic nematodes, Steinernema carpocapsae (Weiser) [Breton strain], Steinernema riobrave Cabanillas, Poinar & Raulston [Weslaco strain], Heterorhabditis bacteriophora Poinar [HP88 strain], and Heterorhabditis indica Poinar, Karunakar & David [Coimbatore strain] was examined in the laboratory against two subterranean termites: Reticulitermes flavipes ...

It has long been recognized that chemotaxis is the primary means by which nematodes locate host plants. Nonetheless, chemotaxis has received scant attention. We show that chemotaxis is predicted to take nematodes to a source of a chemo-attractant via the shortest possible routes through the labyrinth of air-filled or water-filled channels within a soil through which the attractant diffuses. The...

Infection with parasitic nematodes is characterized by the induction of a profound type 2 immune response. We have studied the role of glycans in the induction of the skewed type 2 response by antigens of the parasitic nematode Brugia malayi as well as the free-living nematode Caenorhabditis elegans. Lymph node cells from BALB/c mice immunized with soluble extracts of the two nematodes showed d...

in order to identify the plant parasitic nematodes of pulse fields in northern khorasan province, 41 soil and root samples were collected from rhizosphere of pisum sp., phaseoulus sp., lens sp. during 2011 and 2012. the nematodes were extracted by combined sieving and centrifugal-flotation method of jenkins (1969) and transferred to glycerin by using method of de grisse (1969). the permanent sl...