Slices from adipose tissue and liver were incubated with uniformly labeled C14-isoleucine and the respiratory carbon dioxide, fatty acids, and nonsaponifiable lipids were isolated and analyzed for radioactivity. Organic acids were qualitatively identified by chromatography and autoradiography. Adipose tissue oxidized isoleucine to carbon dioxide a t a rate greater than that of liver. Conversion...

Two type of adulterants i.e. soybean oil (SO) and buffalo depot fat (BDF) along with pure cow and buffalo milk fats, collected and prepared after every two months of interval for a complete one year, were analyzed for their fatty acid composition using gas liquid chromatography. Both the adulterants were added individually at 5, 10 and 15 percent levels (v/v) as well as in their combinations at...

In the past, investigators have used thyroxine labeled either with JIP" in the 3',5'-position or with C14 in the carboxyl position to study the metabolism of thyroxine in vivo. Although these studies have yielded much valuable information, our knowledge about the metabolic fate of the ring structure in thyroxine is incomplete because both the 3'5'-IJ13 and the carboxyl-C14 are rapidly removed f...

The endogenous production of unsaturated fatty acids (FA), particularly some monounsaturated FA (%MONO) and nearly all conjugated linoleic acids, is regulated by the 9-desaturase activity. The aims of this study were to assess the variation of this enzymatic activity within lactation, across dairy breeds, and to estimate its genetic parameters. The ratios of C14:1 cis-9 to C14:0, C16:1 cis-9 to...

At 24 hours after administration of a single dose of 3-4 mg. of l-hydroxy-2-acetylaminofluorene-1-C14 (1-hydroxy-AAF-l-C14) intraperitoneally to rats, 75 per cent of the dose had been excreted in the urine and feces. About 5 per cent of the injected radioactivity was present in the gastrointestinal contents, and only 1-2 per cent re mained in the tissues of the rat. Radioactivity equivalent to ...

At 24 hours after administration of a single dose of 3-4 mg. of l-hydroxy-2-acetylaminofluorene-1-C14 (1-hydroxy-AAF-l-C14) intraperitoneally to rats, 75 per cent of the dose had been excreted in the urine and feces. About 5 per cent of the injected radioactivity was present in the gastrointestinal contents, and only 1-2 per cent re mained in the tissues of the rat. Radioactivity equivalent to ...