An investigation has been made about the vesicular arbuscular mycorrhizal fungi colonization in some medicinal plants Catharanthus roseus, C. pusillus, Wrightia tinctoria, Thevetia peruviana, Nerium indicum and Alstonia scholaris belongs to family Apocyanaceae were screened for the occurrence and association of VA mycorrhizal fungi. All the plants screened in the study harbored VA mycorrhizal f...

Background & Aim: Investigation of suspension cell culture and callus production in Catharanthus roseus. Catharanthus roseus is an herbaceous perennial plant belongs to the family Apocynaceae. C. roseus is important as commercial medicinal plant because of its alkaloidal content. Whole plant contains about 130 alkaloids those are classified as vinca alkaloids...

We have found that coupling between catharanthine and vindoline occurs non-enzymatically in the presence of flavin mononucleotide and manganese ions with near-ultraviolet light irradiation in vitro. The present study found that the concentrations of catharanthine and vindoline in Catharanthus roseus decreased and those of dimeric indole alkaloids increased under near-ultraviolet light at 4 degr...

In this study, three semisynthetic betulonic acid-based compounds, 20(29)-dihydrolup-2-en[2,3-d]isoxazol-28-oic acid, 1-betulonoylpyrrolidine, and lupa-2,20(29)-dieno[2,3-b]pyrazin-28-oic acid, were studied in biotransformation experiments using Nicotiana tabacum and Catharanthus roseus cell suspension cultures. Biotransformation was performed using cyclodextrin to aid dissolving poorly water-s...

Catharanthus roseus (L.) G.Don is a plant which produces anticancer and anti-mitotic indole alkaloids. Colchicine is an anti-mitotic drug. Anti-mitotic effects of Vinca herbacea Waldst. & Kit. indole alkaloids is unknown. The study were evaluated the antimitotic effect of alkaloids of V. herbacea, Catharanthus roseus andcolchicineon mitosis and microtubule arrangement of human blood cells. In t...

A comprehensive metabolomic profiling of Catharanthus roseus L. G. Don infected by 10 types of phytoplasmas was carried out using one-dimensional and two-dimensional NMR spectroscopy followed by principal component analysis (PCA), an unsupervised clustering method requiring no knowledge of the data set and used to reduce the dimensionality of multivariate data while preserving most of the varia...

The medicinal plant, Catharanthus roseus, accumulates wide range of terpenoid indole alkaloids, which are well documented therapeutic agents. In this study, deep transcriptome sequencing of C. roseus was carried out to identify the pathways and enzymes (genes) involved in biosynthesis of these compounds. About 343 million reads were generated from different tissues (leaf, flower and root) of C....

Stem bark of Acacia nilotica, Delbergia sissoo, Delonix regia, Polyalthia longifolia, Prosopis cineraria, and Zyziphus mauritiana, and leaves of Catharanthus roseus, Mentha arvensis, Murraya koinigii, Ocimum basilicum, Tabernaemontana divaricata, Tinospora cordifolia and Tribulus terrestris were analyzed for their total phenolic and flavonois content as well as antioxidant activity. Stem bark o...

While this Article was undergoing peer review, Salim et al. (2013), Asada et al. (2013) and Salim et al. (2014) published individual steps of the (seco)iridoid biosynthesis pathway in Catharanthus roseus, including the molecular and biochemical characterization of 7-deoxyloganic acid hydroxylase, 7-deoxyloganetic acid glucosyl transferase and 7-deoxyloganetic acid synthase, which are in agreeme...

Arabinogalactan protein (AGP), a class of cell wall proteoglycan, was isolated from the hairy root cultures of a newly developed hairy root line IIT-BT/D1 of Catharanthus roseus (L.) G. Don. AGP was found to be present both in the roots (0.3 mg/g fresh weight) and in the spent media (47 mg/L). The compositional analysis revealed the predominance of arabinose and galactose sugar, a characteristi...