Background: VHL (von Hippel-Lindau), Runx-3 (Runt-related transcription factor 3), E-cadherin (Epithelial cadherin), P15 (INK4a, cyclin dependent kinase inhibitor), and P16 (INK4b) genes are essential in hematopoiesis. The aim of this study was to explore the correlation between gene expression and promoter methylation in CD34+ stem cells before and after differentiation to erythroid lineage. ...

introduction: hematopoietic stem cells are always in a quiescence state. since they need retroviral transduction to infect dividing cells, they are resistant to retrovirus transduction. they need to be pre-stimulated by a cytokine cocktail. for proliferation without maturation, we suggest mip-1α as a novel factor. material and methods: retroviral vector produced by pg13/ln c8 cells titter on he...

Introduction: The use of cord stem cells, as a cell source for bone marrow reconstruction in patient under hematopoietic stem cell transplantation is enhancing. The amount of CD34+cells collected from cord blood compared to peripheral blood and bone marrow are less, but their collectionprocesure is easier. Delay in freezing has negative effect on cells surveillance and reduce their ability in ...

allogeneic hematopoietic stem cell transplantation is used in the treatment of patients suffering from hematologic and non-hematologic disorders, but the application is limited by the identification of a suitable donor. umbilical cord blood (ucb) is an alternative source of hematopoietic stem cell (hsc) transplantation. despite all advantages, the limited cell dose is one of the  major obstacle...

background: differentiation ofmesenchymal stem cells (mscs) to hepatocyte-like cells could be associated with development of liver function factors. the impact of differentiation-dependent changes on dna integrity is not well understood. in this study, hepatocytes and their progenitor stem cells were treated with aflatoxin b1 (afb1) and amplification of selected genes linked to dna damage was e...

background: the conventional chemotherapeutic regimens which applied for treatment of acute myeloid leukemia (aml) mostly target tumor bulk but not leukemic stem cells (lscs). aberrant expression or activation of mediators such as osteopontin (opn) or pi3k/pten/akt/mtor pathway plays a key role in making prone to develop leukemia. preventing or treating cancer by curcumin (cur) has been suggest...

Background: Umbilical cord blood hematopoietic stem cells (UCB-HSCs) are an attractive source for transplantation. The generation of megakaryocyte-committed cells could lead to shorten period of thrombocytopenia after HSCs transplantation. Platelet lysate (PL) unlike fetal bovine serum (FBS) can prevent immune problems as well as avert transmission of certain diseases to the recipient. In this ...

Objective. Human CD34 2 hematopoietic stem cells (HSCs) have been identified as potential precursors of CD34 1 HSCs by using xenogeneic transplantation systems. However, the properties of CD34 1 cells generated from CD34 2 cells have not been precisely analyzed due to the lack of an in vitro system in which CD34 1 cells are continuously produced from CD34 2 cells. We conducted this study to det...

CD19-directed immunotherapies have revolutionized the treatment of advanced B-ALL. Despite initial impressive rates complete remission (CR) many patients ultimately relapse. The fact that B-ALL successfully treated with T-cells eventually relapse, coupled early onset CD22 expression during B-cell development suggest pre-existing CD34+CD22+CD19- (pre)-leukemic cells could represent an “early pro...