we have analysed data from 150 patients initially classified as having cvid. about 10% had laboratory abnormalities suggesting known single gene disorders (eg: hyper-igm syndrome), and in a few a genetic defect has been confirmed. we have attempted to sub-classify the remaining patients by analysis of their circulating lymphocytes. b lymphocyte markers have been used to estimate the numbers of ...

interleukin (il)-35 is a newly discovered suppressive cytokine and has been shown to alleviate  inflammatory  and  autoimmune  diseases.  the  purpose  of  this  study  was  to investigate immunomodulatory capacity of il-35 in patients with allergic asthma.il-35 mrna expression levels in peripheral blood mononuclear cells (pbmcs) were detected  by  quantitative  real-time  pcr  (qpcr).  the  fr...

CD8 T cells are critical for the clearance of intracellular pathogens. Upon infection, naive CD8 T cells differentiate into effector cells that target and eliminate infected cells. Following clearance of the pathogen, most effector cells die, although a small fraction survives to establish a memory population. Subsequent exposure to the same pathogen induces a rapid response of memory T cells a...

Functional and phenotypic characterization of virus-specific CD8 T cells against cytomegalovirus, Epstein-Barr virus, influenza (flu), and HIV-1 were performed on the basis of the ability of CD8 T cells to secrete IFN-gamma and IL-2, to proliferate, and to express CD45RA and CCR7. Two functional distinct populations of CD8 T cells were identified: (i) dual IFN-gamma/IL-2-secreting cells and (ii...

CD8+ T cells are thought to play an important role in protective immunity to tuberculosis. The major histocompatibility complex class I subtype HLA-A*0201 is one of the most prevalent class I alleles, with a frequency of over 30% in most populations. HLA-A*0201 transgenic, H-2Db/mouse beta2-microglobulin double-knockout mice (HHD) which express human HLA-A*0201 but no mouse class I, was shown t...

The extent to which the progeny of one primary memory CD8 T cell differs from the progeny of one naive CD8 T cell of the same specificity remains an unresolved question. To explore cell-autonomous functional differences between naive and memory CD8 T cells that are not influenced by differences in the priming environment, an experimental model has been developed in which physiological numbers o...

systemic lupus erythematosus (sle) is a chronic autoimmune disease which results in damage to various organs. some animal studies have revealed that activation of toll-like receptors (tlrs) is important in the pathogenesis of sle. in the present study, the percentage of different immune cell subsets in 35 sle patients and 38 control subjects was analyzed by flow cytometry. we also assessed the ...

Although CD8 T cell-mediated immunosuppression has been a well-known phenomenon during the last three decades, the nature of primary CD8 T suppressor cells and the mechanism underlying their generation remain enigmatic. We demonstrated that naive CD8 T cells primed with allogeneic CD40 ligand-activated plasmacytoid dendritic cells (DC)2 differentiated into CD8 T cells that displayed poor second...

Because the chemokine receptor CCR5 is expressed on Th1 CD4(+) cells, it is important to investigate the expression and function of this receptor on other T cells involved in Th1 immune responses, such as Ag-specific CD8(+) T cells, which to date have been only partially characterized. Therefore, we analyzed the expression and function of CCR5 on virus-specific CD8+ T cells identified by HLA cl...