The mixing of mitochondrial DNA (mtDNA) from the donor cell and the recipient oocyte in embryos and offspring derived from somatic cell nuclear transfer (SCNT) compromises genetic integrity and affects embryo development. We set out to generate SCNT embryos that inherited their mtDNA from the recipient oocyte only, as is the case following natural conception. While SCNT blastocysts produced fro...

The mechanisms that underlie the maintenance of and increase in mutant mitochondrial DNA (mtDNA) are central to our understanding of mitochondrial disease. We have therefore developed a technique based on saponin permeabilisation that allows the study of mtDNA synthesis in intact cells. Permeabilisation of cells has been extensively used in an established method both for studying transcription ...

Correspondence: [email protected] Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai , One Gustave L. Levy place, New York, 10029, USA Full list of author information is available at the end of the article Abstract Eukaryotic cells carry two genomes, nuclear (nDNA) and mitochondrial (mtDNA), which are ostensibly decoupled in their replication, segregation and inherita...

Correspondence: [email protected] Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai , One Gustave L. Levy place, New York, 10029, USA Full list of author information is available at the end of the article Abstract Eukaryotic cells carry two genomes, nuclear (nDNA) and mitochondrial (mtDNA), which are ostensibly decoupled in their replication, segregation and inherita...

PURPOSE Cisplatin adducts to nuclear DNA (nDNA) are felt to be the molecular lesions that trigger apoptosis, but the mechanism linking nDNA adduct formation and cell death is unclear. Some literature in the last decade has suggested a possible direct effect of cisplatin on mitochondria independent of nDNA interaction. In this study, we define separately the sequelae of cisplatin interactions wi...

Mutant mitochondrial (mt) DNA variants are related to human disease and have been investigated using cytoplasmic hybrid (cybrid) cells generated from human tumor cells in which mutant mt maintenance depends on the cell line. It is, however, unclear whether human intercellular fusion of non-tumorous cells influences the maintenance of disease-related mutant mt. A preliminary experiment of cell-c...

Colorectal cancer (CRC) is the third leading cause of cancer-related death worldwide, and its occurrence can be ascribed to genetic susceptibility. Mitochondrial DNA 4977-bp (mtDNA 4977), as the most described mtDNA deletion, has been long proposed to be involved in various types of cancers. However, a few studies on mtDNA 4977-bp deletion in Iranian patients with CRC have been reported. The cu...

Biomarkers are used in cancer detection, diagnosis, and prognosis. In cancer, most genetic markers are based on nuclear DNA; mitochondrial DNA (mtDNA) has not been utilized very extensively. This article provides information about using mtDNA alterations as biomarkers to detect different tumor types at early stages of carcinogenesis. Mitochondria play an important role in cellular energy metabo...

Because of the different characteristics of mtDNA compared with nuclear DNA, the application of mtDNA typing and interpretation of results should not in all ways be the same as that routinely practiced for nuclear DNA typing. A higher success rate with typing mtDNA, as opposed to nuclear DNA, from old bones, severely decomposed or charred remains, or single hair shafts is expected and experienc...

Human aging is associated with a significant reduction in muscle mass (sarcopenia) resulting in muscle weakness and functional limitations in the elderly. Sarcopenia has been associated with mitochondrial dysfunction and the accumulation of mtDNA deletions. Resistance training increases muscle strength and size and can increase mitochondrial capacity and decrease oxidative stress in older adult...