Bacterial cell growth and division require coordinated cell wall hydrolysis and synthesis, allowing for the removal and expansion of cell wall material. Without proper coordination, unchecked hydrolysis can result in cell lysis. How these opposing activities are simultaneously regulated is poorly understood. In Mycobacterium tuberculosis, the resuscitation-promoting factor B (RpfB), a lytic tra...

The increase in antibiotic resistance world-wide revitalized the interest in the use of phage lysins to combat pathogenic bacteria. In this work, we analyzed the specific cleavage sites on the staphylococcal peptidoglycan produced by three phage lytic proteins. The investigated cell wall lytic enzymes were the endolysin LysH5 derived from the S. aureus bacteriophage vB_SauS-phi-IPLA88 (phi-IPLA...

EloR is an RNA-binding protein recently discovered to be involved in regulating cell elongation Streptococcus pneumoniae. It has been found localize midcell. Winther et al. (e00691-20) that it the Jag domain of determines this localization. Using a bacterial two-hybrid assay and coimmunoprecipitation, they acts complex with lytic transglycosylase MltG, which crucial for elongating pneumococcal ...

The Bacillus subtilis cell wall binding protein, CwbA, stimulated the cell wall lytic activities of the B. subtilis and B. licheniformis autolysins (CwlA and CwlM, respectively) in addition to that of the major B. subtilis autolysin (CwlB). Even though the substrate for the enzyme reaction was changed from B. subtilis cell wall containing a teichoic acid to Micrococcus luteus cell wall containi...

Densified Wood Films In article number 2205056, Qi Zhou and co-workers show that cellulose-oxidizing enzyme lytic polysaccharide monooxygenase (LPMO) can nanofibrillate cellulose microfibril bundles inside the wood cell wall, altering microstructure. Therefore, delignified be densified under ambient temperature low pressure into transparent, foldable, ultrastrong films. This enzymatic approach ...