The aim of the present work was focused on the cellulase production by Aspergillus niger using different substrates (i.e) rice bran, wheat bran, black gram bran, coconut oil cake, groundnut oil cake, and gingely oil cake. Solid state fermentation holds tremendous potentials for the production of the enzyme cellulase by Aspergillus niger. These agro industrial residues are cheap raw materials fo...

The activity of pectin esterase and cellulase in abscission of citrus explants was studied. No relation was established between pectin esterase and abscission, while cellulase activity was markedly increased before abscission and for a certain period after excision. IAA and cycloheximide delay abscission and cellulase activity, while ethylene and, to a lesser extent, GA(3) accelerate them. Appl...

Bioethanol from cellulosic raw material has proved to be the best alternative renewable energy source. Cellulase is a multienzyme complex catalyses the bioconversion of cellulose to glucose, which can be used for ethanol production. The objective of this research is to reduce the cost of cellulase production by optimization of fermentation conditions and modeling of the fermentation process. Re...

The physiology and anatomy of abscission has been studied in considerable detail; however, information on the regulation of gene expression in abscission has been limited because of a lack of probes for specific genes. We have identified and sequenced a 595 nucleotide bean (Phaseolus vulgaris cv Red Kidney) abscission cellulase cDNA clone (pBACl). The bean cellulase cDNA has extensive nucleic a...

One of the main challenges of using recombinant enzymes is that they are derived from genetically-modified microorganisms commonly located in the intracellular region. The use of these recombinant enzymes for commercial purposes requires the additional processes of cell disruption and purification, which may result in enzyme loss, denaturation, and increased total production cost. In this study...

We evaluated the effects of cellulase (from Trichoderma longibrachiatum) application rates on neutral detergent fiber (NDF) concentration and fermentation products of orchardgrass (Dactylis glomerata L.) and alfalfa (Medicago sativa L.) silages harvested with decreasing dry matter (DM) digestibility. Additionally, the impacts of inoculant (Lactobacillus plantarum and Pediococcus cerevisiae), pe...

The main purpose of this study is to reduce the production cost of cellulase by optimizing the production medium and using an alternative carbon source such as municipal solid waste residue. In the present investigation, we aim to isolate the two novel cellulase producing fungi (Aspergillus niger and Trichoderma sp.) from municipal solid waste. Municipal solid waste residue (4-5% (w/v)) and pep...

Despite the important role of MAPKs in signal transduction, their functions in the cellulase hyper-producing filamentous fungus Hypocrea jecorina haven't been studied except for the Hog1-like Tmk3. In this work, we constructed and explored the features of H. jecorina Δtmk2 to identify the role of this Slt2-homologous Tmk2. It is suggested from the results that Tmk2 is involved in cell wall inte...

A practical, quantitative approach has been designed, which makes it possible to accurately estimate the saccharifying activities of crude cellulase preparations for insoluble cellulosics. The challenge in activity determination imposed by changes in hydrolysis time and concentration of cellulase and cellulosics on the assay could be overcome by selection of the specific conversion percentage o...