An attempt was made to prepare FITC-labeled-lactoferrin (LF-FTC)-loaded microparticles, durable under gastrointestinal conditions, first by the combination of alginate/calcium complexation and emulsification-evaporation and next by treatment with chitosan solution. The obtained microparticles were examined for particle characteristics, in vitro release profiles and physical stability in solutio...

Nanoscale particles offer a variety of interesting properties, and there is growing interest in their assembly into higher ordered structures. We report that the pH-responsive aminopolysaccharide chitosan can mediate the electrodeposition of model nanoparticles. Chitosan is known to electrodeposit at the cathode surface in response to a high localized pH. To demonstrate that chitosan can mediat...

Pervaporation (PV) dehydration of isopropanol (IPA) using modified chitosan membranes was studied. The membranes were prepared from chitosan and were modified by using zeolite-A. Pre-weighed amount of zeolite-A was added into acidic solvent and stirred to produce homogeneous solution. Chitosan flakes were then added into the solution and stirred overnight. Various ratios of zeolite-A and chitos...

OBJECTIVE The study objectives were to (1) assess the efficacy of N,O-carboxymethyl chitosan on postsurgical adhesion formation after cardiac surgery using a rabbit cardiac injury model and (2) explore the mechanism of action of N,O-carboxymethyl chitosan in the prevention of postsurgical adhesions using in vitro experimentation. METHODS In the rabbit cardiac injury model, cardiac injury was ...

We describe a simple way to create patterns of ‘‘soft’’ biomolecular nanostructures such as vesicles on ‘‘hard’’ surfaces such as gold. The key to our approach is the use of an amphiphilic biopolymer as an ‘‘interconnect’’ or tether. The polymer is hydrophobically modified chitosan (hm-chitosan), which is obtained by covalently attaching alkyl tails to the backbone of chitosan. We electrodeposi...

We report the in situ generation of pH gradients in microfluidic devices for biofabrication of freestanding, semi-permeable chitosan membranes. The pH-stimuli-responsive polysaccharide chitosan was enlisted to form a freestanding hydrophilic membrane structure in microfluidic networks where pH gradients are generated at the converging interface between a slightly acidic chitosan solution and a ...

Chitosan is a natural biopolymer that must be dissolved in an acid solution to activate its antimicrobial and eliciting properties. Among 15 acids tested, chitosan dissolved in 1% solutions of acetic, L-ascorbic, formic, L-glutamic, hydrochloric, lactic, maleic, malic, phosphorous, and succinic acid. To control gray mold, table grape berries were immersed for 10 s in these chitosan solutions th...

The crystallization of calcium carbonate (CaCO3) was investigated using a mineralization system composed of a chitosan membrane and regenerated silk fibroin (RSF). Such a system may resemble the mineralization in molluscs, where chitosan is a derivative of chitin and RSF an analogue of nacreous protein. It was found that the vaterite disks generally formed on the chitosan membrane while the ara...

While microcantilevers offer exciting opportunities for mechano-detection, they often suffer from limitations in either sensitivity or selectivity. To address these limitations, we electrodeposited a chitosan film onto a cantilever surface and mechano-transduced detection events through the chitosan network. Our first demonstration was the detection of nucleic acid hybridization. In this instan...

An ideal scaffold for bone regeneration will provide adequate mechanical support and eventually degrade after bone healing without causing harm to the body. Chitosan, mixed with carboxymethyl chitosan, creates a scaffold with high porosity, but low mechanical strength. Therefore, the objective of this research is to develop biofunctional chitosan based scaffolds for possible application in bone...