Choline and glycine are inter-related through their roles in methyl metabolism. Choline is metabolized to betaine, which donates a methyl group to homocysteine to form methionine, also generating dimethylglycine, which is further metabolized to glycine. Choline is transported across the placenta and is higher in fetal than maternal plasma. Placental glycine transfer, however, is limited and poo...

The construction and performance of an amperometric microbiosensor for the determination of choline chloride by immobilizing choline oxidase on a cellulose acetate membrane are described. The procedure of choline determination involved the electrochemical detection of enzymatically generated hydrogen peroxide. This microsensor has a linear response between 0.038-0.1 mM choline chloride. The...

Cultures of embryonic chick muscle cells grown in medium containing phospholipase C from Clostridium perfringens incorporated [‘Hlcholine into lipid at a rate 3to &fold higher than control cultures. To determine the mechanism by which stimulation of phosphatidylcholine synthesis occurred in phospholipase C-treated cells, activities of enzymes and levels of intermediates in the biosynthetic path...

Mycobacterium avium complex (MAC) is the most common non-tuberculous mycobacterium (NTM) and causes different types of pulmonary diseases. While genomic transcriptomic analysis 104 (M. 104) has been extensive, little known about proteomics M. 104. We utilized technology to analyze changes in whole proteome during exponential stationary growth phases. found 12 dys-regulated proteins; up-regulate...

1. The choline dehydrogenase (EC 1.1.99.1) WAS SOLUBILIZED FROM ACETONE-DRIED POWDERS OF RAT LIVER MITOCHONDRIA BY TREATMENT WITH Naja naja venom. 2. The kinetics of the reaction of enzyme with phenazine methosulphate and ubiquinone-2 as electron acceptors were investigated. 3. With both electron acceptors the reaction mechanism appears to involve a free, modified-enzyme intermediate. 4. With s...

Effects of different levels of choline and garlic powder on performance, liver health and immune response in broiler chickens were evaluated in this study. A factorial experiment with 2 dietary choline levels (the recommended level in Ross 308 broiler guidelines and 25 % less than the recommendation) and 3 dietary garlic powder levels (0, 5 or 10 g/kg diet) was conducted using Ross 308 broiler ...

The increased oxygen uptake of liver suspensions on the addition of acetylcholine, which was reported by Bernheim and Bernheim in 1933 (2), was shown by Mann and Quastel (3) to be due to the oxidation of choline. Betaine aldehyde was identified as the oxidation product (3). The choline oxidase (CO) system was found by Marm, Woodward, and Quastel (4) to consist partly of a typical dehydrogenase....

We now continued our search in the 609 bp 3'-UTR region of GATA4 to explore further molecular avenues leading to CHD. Background: To identify further genetic alterations associated with CHD, we continued our search in the 609 bp of 3'-untranslated region (3'-UTR) of GATA4. 2. The authors should modify Table 3, indicating with a different character (for example in bold) the new sequence variatio...