An enzyme purified from squid nerve that hydrolyzes the cholinesterase inhibitor diisopropyl phosphorofluoridate (DFP) has now been coupled to agarose beads. A column of this agarose-DFPase hydrolyzes the nerve gas 1,2,2-trimethylpropyl methylphosphonofluoridate (Soman). Although the more inhibitory of the four diastereoisomers of Soman are hydrolyzed least rapidly, a column of sufficient lengt...

This report assesses the value of cholinesterase (ChE) activity as an indicator of liver function and analyses its usefulness in the diagnosis of jaundice. A rapid method of determination has been used, and alterations in ChE activity in conditions other than liver disease have been studied to permit a fuller and more accurate survey of its value as a liver function test. The level of serum cho...

BACKGROUND Plasma cholinesterase activity is known to be correlated with plasma triglycerides, HDL- and LDL-cholesterol, and other features of the metabolic syndrome. A role in triglyceride metabolism has been proposed. Genetic variants that decrease activity have been studied extensively, but the factors contributing to overall variation in the population are poorly understood. We studied plas...

in this study, a commercially available kit in which propionylthiocholine is used as a substrate and a dibucaine solution to determine the variants of the enzyme were utilized. plasma cholinesterase activity of 151 patients who underwent different operations was measured during a period of six months. the mean±sd of the enzyme activity in 150 patients was 4.39±1.17 ku/l .. seventeen patients (1...

There is growing evidence for the assumption that the profound biological effects produced by some compounds in low concentrations may be attributed to an interaction with enzymes. This is true for certain cell constituents as well as for toxic compounds not existing normally in the body. Several vitamins have been found to be prosthetic groups of enzymes and for a number of drugs it could be d...

There is growing evidence for the assumption that the profound biological effects produced by some compounds in low concentrations may be attributed to an interaction with enzymes. This is true for certain cell constituents as well as for toxic compounds not existing normally in the body. Several vitamins have been found to be prosthetic groups of enzymes and for a number of drugs it could be d...

The kinetic characteristic (Km) of cholinesterase from the crab Chasmagnathus granulata, the shrimp Farfantepenaeus paulensis and the fish Odontesthes bonaeriensis were compared and correlated with the anticholinesterasic effect of eserine (physostigmine). For the crustaceans, the estimated Km values were about 5-8 times higher than that estimated for the fish (0.04 mM). In the crab and the shr...

Enzymes hydrolysing acetylcholine have attracted much attention because acetylcholine is concemed with the functioning of the nervous system. The extensive literature on cholinesterases has been reviewed at intervals (Zeller, 1948; Koelle & Gilman, 1949; Augustinsson, 1950; Nachmansohn & Wilson, 1951; Whittaker, 1951; Davies, 1954; Bergmann, 1958; Davies & Green, 1958). Although there is ample ...

Purpose: Cholinesterase inhibitors are the class of compounds which inhibit cholinesterase enzyme. These are used as drugs for symptomatic treatment of Alzheimer’s disease (AD). The present study, evaluate antibutyrylcholinestease property of ethanolic extract of Shorea robusta de oil cake (DOC). Method: Ellman’s method was used to determine the butyrylcholinesterase (BuChE) enzyme inhibitory a...

Redistribution of axonal enzymes as a function of time in vitro was studied in an unbranched segment of frog sciatic nerve. Cholinesterase activity moved peripherally at a rate of 99 mm/day and centrally at 19 mm/day. One-quarter of the total nerve content of the enzyme was estimated to be in motion, one-eighth in each direction. Mitochondrial enzymes (hexokinase and glutamic dehydrogenase) mov...